Havrdová J, Bláha K, Tichý P, Rosina J, Cikrt M
Institute of Hygiene and Epidemiology, Praha, Czechoslovakia.
J Hyg Epidemiol Microbiol Immunol. 1987;31(3):245-50.
The distribution of 110mAg in the elution fractions obtained from gel filtration (Sephadex G-75) of the supernatants of rat liver homogenates was studied. 110mAgNO3 was administered i.v. at the doses 0.183 and 0.912 mg Ag+ . kg-1 . b . wt.and rats were killed 30 min after the administration. Differential centrifugation of the homogenates was performed to determine 110mAg+ distribution in subcellular structures of hepatocytes. The effect of CdCl2 and AgNO3 pretreatment (2 s.c. injections of 2.5 mg . kg-1 during 48 h) was investigated and results were compared with those obtained without pretreatment. It was found that AgNO3 pretreatment does not affect 110mAg+ distribution in the elution fractions and the major part of the metal is concentrated in the high-molecular-weight protein fraction. On contrary, after CdCl2 pretreatment almost all 110mAg+ is bound in the metallothionein (MT) fraction. Differential centrifugation revealed the main portion of the metal in nuclei and cell membranes and only small amount in lysosomal supernatant. After CdCl2 pretreatment the content of 110mAg+ in supernatant considerably increases. Results confirm the affinity of silver to MT and moreover show that this metal probably does not significantly induce the MT synthesis in the liver tissue.
研究了从大鼠肝脏匀浆上清液经凝胶过滤（葡聚糖凝胶G - 75）获得的洗脱级分中¹¹⁰mAg的分布。以0.183和0.912 mg Ag⁺·kg⁻¹·bw的剂量静脉注射¹¹⁰mAgNO₃，给药后30分钟处死大鼠。对匀浆进行差速离心以确定¹¹⁰mAg⁺在肝细胞亚细胞结构中的分布。研究了CdCl₂和AgNO₃预处理（在48小时内皮下注射2次，每次2.5 mg·kg⁻¹）的效果，并将结果与未预处理的情况进行比较。发现AgNO₃预处理不影响洗脱级分中¹¹⁰mAg⁺的分布，且大部分金属集中在高分子量蛋白质级分中。相反，CdCl₂预处理后，几乎所有的¹¹⁰mAg⁺都结合在金属硫蛋白（MT）级分中。差速离心显示金属主要分布在细胞核和细胞膜中，溶酶体上清液中含量较少。CdCl₂预处理后，上清液中¹¹⁰mAg⁺的含量显著增加。结果证实了银与MT的亲和力，而且表明这种金属可能不会显著诱导肝脏组织中MT的合成。
