Ichesco W R, Ellison R L, Corcoran J F, Krause D C
Department of Endodontics, University of Michigan School of Dentistry, Ann Arbor.
J Endod. 1991 Oct;17(10):503-7. doi: 10.1016/S0099-2399(06)81799-1.
The purpose of this study was to compare dye leakage in the dentin of resected and nonresected roots. Fifty-four single rooted extracted teeth were used. The two groups were subdivided into young, middle age, and old age samples. Each sample was biomechanically prepared in a standard fashion and obturated using lateral condensation with sealer and gutta-percha. All root surfaces were sealed with nail polish, leaving the apical portion exposed. The apical portion of the roots were placed in 2% methylene blue dye for 72 h, rinsed, and placed in 15 ml of 35% HNO3 for 72 h. The supernatant was analyzed at 640 nm using a visible light spectrophotometer. The amount of leakage was extrapolated from a standard linear regression curve constructed from the stock 2% methylene blue dye solution. The percentage of concentration leakage in all samples ranged from less than 0.0600 to 0.1658. It was concluded that there is a greater amount of leakage in resected versus nonresected extracted teeth. The data also suggest that older teeth exhibit less leakage than younger teeth.
本研究的目的是比较切除牙根与未切除牙根牙本质中的染料渗漏情况。使用了54颗单根拔除牙。两组又细分为年轻、中年和老年样本。每个样本均以标准方式进行生物力学预备，并用封闭剂和牙胶尖进行侧方加压充填。所有牙根表面均用指甲油密封，仅使根尖部分暴露。将牙根的根尖部分置于2%亚甲蓝染料中72小时，冲洗后再置于15毫升35%硝酸中72小时。使用可见光分光光度计在640纳米波长处分析上清液。渗漏量通过由储备的2%亚甲蓝染料溶液构建的标准线性回归曲线外推得出。所有样本中浓度渗漏的百分比范围为小于0.0600至0.1658。得出的结论是，与未切除的拔除牙相比，切除牙的渗漏量更大。数据还表明，老年牙齿的渗漏比年轻牙齿少。
