Frasch C E, Sacchi C T, Brandiolone M C, Vieiera V S, Leite L C
Center for Biologics Evaluation and Research, Division of Bacterial Products, Bethesda, Maryland.
NIPH Ann. 1991 Dec;14(2):225-30; discussion 230-1.
Outer membrane protein vaccines have now been demonstrated to be effective in prevention of group B Neisseria meningitidis disease, but the extent and duration of protection needs improvement. To develop a second generation outer membrane protein vaccine we have begun studies to evaluate vaccines containing iron regulated proteins in lipopolysaccharide depleted outer membrane vesicles. Since the iron regulated proteins are critical for in vivo survival, it is our working hypothesis that antibodies to these proteins will either be bactericidal or block iron uptake. Four representative strains were selected, and preliminary studies indicate that vaccines containing the iron regulated proteins are immunogenic in mice.
外膜蛋白疫苗现已被证明在预防B群脑膜炎奈瑟菌疾病方面有效，但保护的程度和持续时间仍需改进。为了开发第二代外膜蛋白疫苗，我们已开始研究，以评估在脂多糖缺失的外膜囊泡中含有铁调节蛋白的疫苗。由于铁调节蛋白对体内生存至关重要，我们的工作假设是，针对这些蛋白的抗体将具有杀菌作用或阻止铁的摄取。我们选择了4株代表性菌株，初步研究表明，含有铁调节蛋白的疫苗在小鼠中具有免疫原性。
