Califano J V, Schenkein H A, Tew J G
Medical College of Virginia, Richmond.
Oral Microbiol Immunol. 1991 Aug;6(4):228-35. doi: 10.1111/j.1399-302x.1991.tb00482.x.
This study was undertaken to examine the characteristics of the immunodominant antigens of Actinobacillus actinomycetemcomitans serotypes a and c. The top responders for A. actinomycetemcomitans serotypes a and c were selected (19 for serotype a and 21 for serotype c) from 150 clinically characterized patients. Competition assays revealed that 9 of 19 of these patients were reacting specifically to serotype a and 12 of 21 for serotype c. Limiting dilution analysis on Western blots revealed that most antigen bands apparent at low dilution disappeared as the patient's serum was diluted. The antigen band(s) remaining at the endpoint or the dilution corresponding to the antibody titer were defined as immunodominant. For serotype a there were several different immunodominant antigens but none was present in more than half of the subjects. For serotype c the immunodominant antigens included a number of discrete bands and a diffuse smeared polysaccharide band. Only 2 of these antigens were present in the majority of the high-responders: 92% had the smeared antigen and 67% had a 15 kDa antigen. The 15 kDa band was a protein common to all A. actinomycetemcomitans serotypes. The smeared antigen was unaffected by protease K treatment and gave a reaction of identity with the serotype c specific rabbit antiserum. This rabbit antiserum is specific for a mannan carbohydrate and does not react with LPS (23). Therefore, the smeared immunodominant antigen appears to be a polysaccharide containing mannan.
本研究旨在检测伴放线放线杆菌血清型a和c免疫显性抗原的特征。从150例具有临床特征的患者中挑选出对伴放线放线杆菌血清型a和c反应最强的患者（血清型a为19例，血清型c为21例）。竞争试验显示，这些患者中，血清型a的19例中有9例对血清型a有特异性反应，血清型c的21例中有12例对血清型c有特异性反应。对蛋白质免疫印迹进行有限稀释分析显示，随着患者血清稀释，低稀释度下明显的大多数抗原条带消失。在终点或与抗体滴度相对应的稀释度下仍存在的抗原条带被定义为免疫显性抗原。对于血清型a，有几种不同的免疫显性抗原，但在超过半数的受试者中均未出现。对于血清型c，免疫显性抗原包括一些离散条带和一条弥散的多糖条带。这些抗原中只有2种存在于大多数高反应者中：92%的人有弥散抗原，67%的人有15 kDa抗原。15 kDa条带是所有伴放线放线杆菌血清型共有的一种蛋白质。弥散抗原不受蛋白酶K处理的影响，与血清型c特异性兔抗血清呈同一性反应。这种兔抗血清对甘露聚糖碳水化合物具有特异性，不与脂多糖发生反应（23）。因此，弥散的免疫显性抗原似乎是一种含甘露聚糖的多糖。
