Suzuki N, Nakano Y, Yoshida Y, Ikeda D, Koga T
Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan.
J Clin Microbiol. 2001 May;39(5):2002-5. doi: 10.1128/JCM.39.5.2002-2005.2001.
Oligonucleotide primers specific for gene clusters involved in the biosynthesis of serotype-specific polysaccharide antigens were designed to identify Actinobacillus actinomycetemcomitans serotypes a to e using the multiplex PCR. This method may be useful for serotype-specific genotyping rapidly and directly from clinical samples containing various organisms.
设计了针对与血清型特异性多糖抗原生物合成相关基因簇的寡核苷酸引物,以使用多重聚合酶链反应(multiplex PCR)鉴定伴放线放线杆菌血清型a至e。该方法对于直接从含有多种微生物的临床样本中快速进行血清型特异性基因分型可能有用。