Holm A, Kalfas S
Department of Oral Microbiology, School of Dentistry, University of Lund, Malmö, Sweden.
Oral Microbiol Immunol. 1991 Aug;6(4):236-40. doi: 10.1111/j.1399-302x.1991.tb00483.x.
Laboratory strains and fresh isolates of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus were examined for cell surface hydrophobicity and for electrokinetic properties under different experimental conditions. Fresh isolates of A. actinomycetemcomitans and H. aphrophilus were hydrophobic. Laboratory strains of A. actinomycetemcomitans were 20-30% less hydrophobic than fresh isolates. No difference was observed between laboratory and fresh isolates of H. aphrophilus. The pH of the suspending buffer, growth phase or incubation atmosphere did not significantly affect the hydrophobicity of the 2 species, whereas agar cultures of H. aphrophilus were less hydrophobic than broth cultures. All A. actinomycetemcomitans strains treated with sterile filtered saliva showed a concentration-dependent decrease in hydrophobicity of at most 30%. H. aphrophilus strains were not affected by the same treatment. Laboratory strains of H. aphrophilus were more negatively charged than A. actinomycetemcomitans, whereas fresh isolates of the 2 species exhibited similar surface charge. In the presence of saliva the mean cell surface charge of laboratory strains decreased by 56% for A. actinomycetemcomitans and by 73% for H. aphrophilus. The results indicate that the 2 species differ in expression of cell structures accounting for hydrophobicity and surface charge and that environmental factors might differently influence the physical properties of the two species analyzed.
对伴放线放线杆菌和嗜沫嗜血杆菌的实验室菌株及新鲜分离株在不同实验条件下的细胞表面疏水性和电动特性进行了检测。伴放线放线杆菌和嗜沫嗜血杆菌的新鲜分离株具有疏水性。伴放线放线杆菌的实验室菌株疏水性比新鲜分离株低20%-30%。嗜沫嗜血杆菌的实验室菌株和新鲜分离株之间未观察到差异。悬浮缓冲液的pH值、生长阶段或培养气氛对这两种菌的疏水性没有显著影响，而嗜沫嗜血杆菌的琼脂培养物比肉汤培养物疏水性更低。所有用无菌过滤唾液处理的伴放线放线杆菌菌株疏水性最多降低30%，呈浓度依赖性。嗜沫嗜血杆菌菌株不受相同处理的影响。嗜沫嗜血杆菌的实验室菌株比伴放线放线杆菌带更多负电荷，而这两种菌的新鲜分离株表现出相似的表面电荷。在唾液存在的情况下，伴放线放线杆菌实验室菌株的平均细胞表面电荷减少56%，嗜沫嗜血杆菌减少73%。结果表明，这两种菌在决定疏水性和表面电荷的细胞结构表达上存在差异，并且环境因素可能对所分析的这两种菌的物理特性产生不同影响。
