Meyer D H, Fives-Taylor P M
Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, College of Medicine, University of Vermont, Burlington 05405.
Infect Immun. 1994 Mar;62(3):928-35. doi: 10.1128/iai.62.3.928-935.1994.
Actinobacillus actinomycetemcomitans smooth variants [SUNY 75(S), SUNY 465, 652] were investigated for their ability to adhere to KB epithelial cells. Both the type of medium (broth versus agar) and anaerobicity influenced adherence levels and cell surface characteristics. Optimal adherence was observed with all three strains after growth of the bacterial cells in broth under anaerobic conditions, a condition which was associated with extracellular microvesicles. Adherence of SUNY 75(S) also was correlated with extracellular amorphous material, whereas adherence of SUNY 465 was also associated with fimbriation which accompanied a smooth to rough phenotype shift. The relationship between adherence and extracellular vesicles, extracellular amorphous material, and fimbriation suggests that all of these components may function in A. actinomycetemcomitans adherence to epithelial cells. The phenotype shift observed in SUNY 465 cells is further evidence that A. actinomycetemcomitans SUNY 465 is predisposed to variant shifts which are associated with changes in adherence and invasion properties.
研究了伴放线放线杆菌光滑变种[SUNY 75(S)、SUNY 465、652]黏附KB上皮细胞的能力。培养基类型（肉汤培养基与琼脂培养基）和厌氧性均会影响黏附水平和细胞表面特征。在厌氧条件下于肉汤培养基中培养细菌细胞后，观察到所有三株菌株均出现最佳黏附情况，此条件与细胞外微泡有关。SUNY 75(S)的黏附还与细胞外无定形物质相关，而SUNY 465的黏附也与菌毛形成有关，菌毛形成伴随着光滑到粗糙的表型转变。黏附与细胞外囊泡、细胞外无定形物质以及菌毛形成之间的关系表明，所有这些成分可能在伴放线放线杆菌黏附上皮细胞过程中发挥作用。在SUNY 465细胞中观察到的表型转变进一步证明，伴放线放线杆菌SUNY 465易于发生变种转变，这些转变与黏附及侵袭特性的变化相关。
