Marigo M, Ferrara S D, Tedeschi L
Arch Toxicol. 1977 Jun 18;37(2):107-12. doi: 10.1007/BF00293859.
The Authors present a gas-chromatographic method which allows for the rapid identification and assay of barbiturates and antiepileptics in body fluids. The Kupferberger procedure for extraction is used. The identification is obtained on two different columns: 10% Dexil 300 GC on Chromosorb W-HP and 3% OV 17 on Chromosorb G AW-DMCS (80-100 mesh). A considerable and interesting reduction of the barbiturates adsorption in the columns is obtained by preliminary conditioning with tetraethylorthosilicate. In this way, phenobarbital and cyclobarbital may also be assayed up to 1 microgram/ml in blood. For the simultaneous assay of therapeutic levels of phenobarbital and hydrantoins methylderivates are better employed: blood concentrations as low as 0.1 microgram/ml are easily detected.
作者提出了一种气相色谱法，可用于快速鉴定和测定体液中的巴比妥类药物和抗癫痫药物。采用库普费尔贝格尔萃取法。在两根不同的色谱柱上进行鉴定：Chromosorb W-HP上的10% Dexil 300 GC和Chromosorb G AW-DMCS（80-100目）上的3% OV 17。通过用原硅酸四乙酯进行预处理，可显著且有趣地减少巴比妥类药物在色谱柱中的吸附。通过这种方式，血液中苯巴比妥和环巴比妥的测定浓度可达1微克/毫升。对于同时测定苯巴比妥和乙内酰脲甲基衍生物的治疗水平，更好的方法是：血液浓度低至0.1微克/毫升也能轻松检测到。
