Slayter H S, Wold J K, Midtvedt T
Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, MA 02115.
Carbohydr Res. 1991 Dec 30;222:1-9. doi: 10.1016/0008-6215(91)89001-v.
Purified germ-free rat intestinal mucin was found by chemical analysis to contain 25% protein, enriched in serine, threonine, and proline, 75% carbohydrate, and no nucleic acid. It was analyzed by darkfield electron microscopy and found to consist of long filamentous molecules with a maximum length of approximately 740 nm, a mean length of 456 nm, and a mean width of 7 nm. Given reasonable assumptions derived from earlier work on other well-characterized mucins, the molecular weight of the peptide, calculated by the length from electron microscopy, was 200,000, and, given the chemical composition, the molecular weight of the entire mucin molecule was calculated to be approximately 800,000.
通过化学分析发现，纯化的无菌大鼠肠道粘蛋白含有25%的蛋白质，富含丝氨酸、苏氨酸和脯氨酸，75%的碳水化合物，且不含核酸。通过暗视野电子显微镜分析发现，它由长丝状分子组成，最大长度约为740纳米，平均长度为456纳米，平均宽度为7纳米。根据早期对其他特征明确的粘蛋白的研究得出的合理假设，通过电子显微镜下的长度计算，该肽的分子量为200,000，根据化学成分计算，整个粘蛋白分子的分子量约为800,000。
