Slomiany A, Okazaki K, Tamura S, Slomiany B L
Research Center, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Arch Biochem Biophys. 1991 May 1;286(2):383-8. doi: 10.1016/0003-9861(91)90055-n.
Human and rat intestinal mucin was purified by equilibrium density gradient centrifugation and Sepharose 2B chromatography according to M. Mantle, D. Mantle, and A. Allen (1981, Biochem. J. 195, 277-285) and analyzed using mucin, DNA, and fibronectin-specific antibodies in dot-blot, ELISA, and Western blotting. The 118-kDa component of the mucins and the 118-kDa fragment of fibronectin from the same source displayed affinity for concanavalin A and immunoreacted with fibronectin antibodies. The amino acid and carbohydrate compositions of the 118-kDa peptide electroeluted by gel electrophoresis of mucin and fibronectin preparations were identical within each pair of glycopeptides and closely resembled the "link protein component" of human and rat intestinal mucin preparations of R. E. F. Fahim, R. D. Specian, G. G. Forstner, and J. F. Forstner (1987, Biochem. J. 243, 631-640) and M. Mantle and G. Stewart (1989, Biochem. J. 259, 631-640). We therefore conclude that the "link protein" claimed to be an integral part of mucus glycoproteins in actuality is the 118-kDa fragment of fibronectin.
根据M. Mantle、D. Mantle和A. Allen（1981年，《生物化学杂志》195卷，277 - 285页）的方法，通过平衡密度梯度离心和琼脂糖2B层析对人和大鼠肠道粘蛋白进行纯化，并使用粘蛋白、DNA和纤连蛋白特异性抗体通过斑点印迹、酶联免疫吸附测定和蛋白质印迹法进行分析。来自同一来源的粘蛋白的118 kDa组分和纤连蛋白的118 kDa片段对伴刀豆球蛋白A显示出亲和力，并与纤连蛋白抗体发生免疫反应。通过对粘蛋白和纤连蛋白制剂进行凝胶电泳电洗脱得到的118 kDa肽段的氨基酸和碳水化合物组成，在每对糖肽中是相同的，并且与R. E. F. Fahim、R. D. Specian、G. G. Forstner和J. F. Forstner（1987年，《生物化学杂志》243卷，631 - 640页）以及M. Mantle和G. Stewart（1989年，《生物化学杂志》259卷，631 - 640页）报道的人和大鼠肠道粘蛋白制剂中的“连接蛋白组分”非常相似。因此，我们得出结论，声称是粘液糖蛋白组成部分的“连接蛋白”实际上是纤连蛋白的118 kDa片段。
