通过直接植入正常人外周血和脐带白细胞在仓鼠体内产生淋巴瘤。

Production of lymphoid tumors in hamsters by direct implantation of normal human peripheral and umbilical cord leukocytes.

作者信息

Miyoshi I, Kubonishi I, Uchida H, Hiraki S, Matsuda Y, Tanaka T, Masuji H, Hiraki K

出版信息

Int J Cancer. 1976 Jul 15;18(1):67-75. doi: 10.1002/ijc.2910180110.

Abstract

Peripheral leukocytes from Epstein-Barr virus (EBV)-seropositive or seronegative normal adults, or human umbilical cord leukocytes infected or not infected with EBV, were directly transplanted into 50 newborn hamsters treated with anti-lymphocyte serum. Lymphoid tumors were produced after a latent period of 10-25 days in 39 animals: 8 of 14 recipients of seropositive donor leukocytes, 2 of 2 recipients of seronegative donor leukocytes, 13 of 15 recipients of EBV-infected cord leukocytes, and 16 of 19 recipients of non-infected cord leukocytes. EBV-determined nuclear antigen-positive human lymphoblastoid cell lines were established in vitro from tumors produced with seropositive donor leukocytes or EBV-infected cord leukocytes but not from tumors produced with seronegative donor leukocytes or non-infected cord leukocytes. The results indicate that EBV is not a prerequisite for in vivo production of tumors but necessary for in vitro establishment of lymphoblastoid cell lines from such tumors.

摘要

将来自爱泼斯坦 - 巴尔病毒（EBV）血清反应阳性或阴性的正常成年人的外周血白细胞，或感染或未感染EBV的人脐带血白细胞，直接移植到50只接受抗淋巴细胞血清治疗的新生仓鼠体内。39只动物在10 - 25天的潜伏期后产生了淋巴瘤：14只接受血清反应阳性供体白细胞的受体中有8只，2只接受血清反应阴性供体白细胞的受体中有2只，15只接受EBV感染脐带血白细胞的受体中有13只，19只接受未感染脐带血白细胞的受体中有16只。用血清反应阳性供体白细胞或EBV感染的脐带血白细胞产生的肿瘤在体外建立了EBV决定的核抗原阳性的人淋巴母细胞系，但用血清反应阴性供体白细胞或未感染脐带血白细胞产生的肿瘤则未建立。结果表明，EBV不是体内产生肿瘤的先决条件，但对于从这类肿瘤体外建立淋巴母细胞系是必要的。