Huberman E, Yamasaki H, Sachs L
Int J Cancer. 1976 Jul 15;18(1):76-82. doi: 10.1002/ijc.2910180111.
Aryl hydrocarbon (benzo(a)pyrene) hydroxylase induced by dibutyryl cyclic AMP (dcAMP), plus aminophylline (AHH I) can be ditsinguished from the hydroxylase induced by benz (a) anthracene (AHH II) by its lower Km for benzo (a) pyrene. Treatment with the combination of benzo (a) anthracene and dcAMP plus aminophylline induced both AHH I and AHH II activities. After optimal induction of AHH II activity by benz (a) anthracene, the addition of dcAMP plus aminophylline gave an induction of AHH I. Although AHH I activity declined to an almost basal level 24 h after treatment with dcAMP plus aminophylline, the addition of benz (a) anthracene prevented this decline. Inducibility by dcAMP plus aminophylline or by benz (a) anthracene varied in different cell lines. Some cell lines were induced by both substances, with a higher induction by benz (a) anthracene, while other lines were inducible only by benz (a) anthracene, and a third cell type was not inducible by either. Selection for resistance to benzo (a) pyrene of a cell line inducible by both compounds resulted in a fourth cell type which was more inducible by dcAMP plus aminophylline than by benz (a) anthracene. The results suggest that there is an independent regulation of hydroxylase AHH I and AHH II and that the induction of these two enzyme activities is determined by different genetic controls.
由二丁酰环磷腺苷（dcAMP）加氨茶碱诱导产生的芳烃（苯并(a)芘）羟化酶（AHH I），因其对苯并(a)芘的米氏常数较低，可与由苯并(a)蒽诱导产生的羟化酶（AHH II）区分开来。用苯并(a)蒽与dcAMP加氨茶碱联合处理可诱导AHH I和AHH II两种活性。在用苯并(a)蒽最佳诱导AHH II活性后，添加dcAMP加氨茶碱可诱导出AHH I。尽管在用dcAMP加氨茶碱处理24小时后AHH I活性降至几乎基础水平，但添加苯并(a)蒽可防止这种下降。dcAMP加氨茶碱或苯并(a)蒽的诱导能力在不同细胞系中有所不同。一些细胞系可被这两种物质诱导，且苯并(a)蒽的诱导作用更强，而其他细胞系仅可被苯并(a)蒽诱导，还有第三种细胞类型对两者均无诱导作用。对两种化合物均可诱导的细胞系进行抗苯并(a)芘筛选，得到了第四种细胞类型，其对dcAMP加氨茶碱的诱导作用比对苯并(a)蒽的诱导作用更强。结果表明，羟化酶AHH I和AHH II存在独立调控，且这两种酶活性的诱导由不同的遗传控制决定。
