培养细胞中芳烃羟化酶活性与苯并(a)芘代谢活性的关系。
Relationship of aryl hydrocarbon hydroxylase activity to benzo(a)pyrene-metabolizing activity of cells in culture.
作者信息
Diamond L, Baird W M
出版信息
Cancer Lett. 1977 Sep;3(3-4):189-95. doi: 10.1016/s0304-3835(77)95553-7.
PMID:902255
Abstract
The basal level of aryl hydrocarbon hydroxylase (AHH) activity was high in cultures of low passage hamster embryo (HE) cells but AHH inducibility by benz(a)-anthracene (BA) was low; in R72/3 rat liver cells, basal activity was low and inducibility was high. The metabolism of 3H--benzo(a)pyrene was similar in cultures of BA-induced R72/3 cells and uninduced HE cells. Thus, low AHH inducibility may not always be an indication of the cells' absolute hydrocarbonmetabolizing capacity.
摘要
低传代仓鼠胚胎（HE）细胞培养物中芳烃羟化酶（AHH）的基础活性较高，但苯并（a）蒽（BA）对AHH的诱导性较低；在R72/3大鼠肝细胞中，基础活性较低而诱导性较高。在BA诱导的R72/3细胞培养物和未诱导的HE细胞培养物中，3H-苯并（a）芘的代谢情况相似。因此，低AHH诱导性可能并不总是细胞绝对烃代谢能力的指标。
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