Oker-Blom N, Kallio A, Hortling L
Intervirology. 1975;5(6):342-53. doi: 10.1159/000149932.
Morphological alterations were observed after 5-15 serial passages of cells infected with three different strains of avian leukosis virus: OK 10, an A subgroup virus isolated from a natural infection; RAV-1, an established laboratory strain of the A subgroup, and RAV-2, a laboratory strain of the B subgroup. The infected cells had a prolonged lifespan of approximately 28 passages, compared to 14 passages for control cells. However, the altered cells had none of the attributes of transformed cells, such as growth in soft agar, loss of contact inhibition, tumor formation in chickens, or loss of fibroblast surface antigen. Therefore, we refer to the changes as conversion rather than transformation. The morphological changes differed depending upon the subgroup of the inducing virus. Cells converted with the A subgroup viruses were uniformly epitheloid whereas cells converted with the B subgroup virus were less uniform in size and shape. We speculate that an event similar to conversion may take place in vivo and contribute to the oncogenicity of leukosis viruses.
在用三种不同毒株的禽白血病病毒感染细胞并进行5 - 15次连续传代后，观察到了形态学改变。这三种病毒分别是：OK 10，一种从自然感染中分离出的A亚群病毒；RAV - 1，一种已建立的A亚群实验室毒株；以及RAV - 2，一种B亚群实验室毒株。与对照细胞的14次传代相比，被感染的细胞具有约28次传代的延长寿命。然而，这些改变的细胞不具备转化细胞的任何特征，如在软琼脂中生长、接触抑制丧失、在鸡体内形成肿瘤或成纤维细胞表面抗原丧失。因此，我们将这些变化称为转化而非转变。形态学变化因诱导病毒的亚群而异。用A亚群病毒转化的细胞均匀地呈上皮样，而用B亚群病毒转化的细胞在大小和形状上则不太均匀。我们推测，类似转化的事件可能在体内发生，并有助于白血病病毒的致癌性。
