Arshad S S, Howes K, Barron G S, Smith L M, Russell P H, Payne L N
Institute for Animal Health, Compton, Nr Newbury, Berkshire, UK.
Vet Pathol. 1997 Mar;34(2):127-37. doi: 10.1177/030098589703400205.
The tissue tropism was studied for the HPRS-103 strain of avian leukosis virus, which belongs to a new envelope subgroup, designated J. Studies were conducted in blood monocyte and bone marrow cell cultures and in chickens from six lines that had been shown previously to differ in susceptibility to induction by this virus of myeloid leukosis and other tumors. Using an immunohistochemical technique to detect expression of viral group-specific antigen (Gag) in various tissues, we detected no major differences among the six lines of chickens at 3 and 7 weeks of age following infection as embryos. Thus, Gag expression did not correlate with differences in tumor susceptibility. Of the tissues examined, greatest Gag expression was observed in cells specific to the adrenal gland, heart, kidney, proventriculus and especially in smooth muscle cells and connective tissue. After infection of 1-day-old chicks, greater tissue expression was observed in line 21 chicks, which mostly developed a tolerant viremic infection, than in Brown Leghorn chicks, which developed virus-neutralizing antibodies. An acutely transforming virus, strain 966, derived from HPRS-103-induced myeloid leukosis, showed a tropism similar to HPRS-103. The HPRS-103 strain showed a lower propensity to replicate in the medullary region of the lymphoid follicles of the bursa of Fabricius than did the RAV-1 strain of subgroup A avian leukosis virus. This low bursal tropism may be a factor in why HPRS-103 does not induce lymphoid leukosis. The HPRS-103 and 966 virus replicated in blood monocyte cultures from chickens from the six lines, indicating a tropism for the myelomonocytic cell lineage. In comparison, as previously reported, RAV-1 did not replicate well in the monocyte cultures, whereas RAV-2, a subgroup B avian leukosis virus, did replicate. The tropism of HPRS-103 for monocytes may relate to its ability to cause myeloid leukosis. Monocyte and bone marrow cell cultures from the six lines ranked similarly in differences in susceptibility to transformation by 966 virus and showed evidence that their relative susceptibilities correlated with susceptibility of chickens from these lines to induction of myeloid leukosis by HPRS-103, suggesting common tissue-specific viral and host factors involved in oncogenesis by these two viruses.
对属于新的包膜亚群（命名为J）的禽白血病病毒HPRS - 103株的组织嗜性进行了研究。研究在血液单核细胞和骨髓细胞培养物中以及在先前已证明对该病毒诱导的髓细胞性白血病和其他肿瘤的易感性存在差异的六个品系的鸡中进行。使用免疫组织化学技术检测各种组织中病毒群特异性抗原（Gag）的表达，我们发现在胚胎期感染后3周和7周龄的六个品系鸡之间没有主要差异。因此，Gag表达与肿瘤易感性差异无关。在所检查的组织中，在肾上腺、心脏、肾脏、腺胃特有的细胞中观察到最高的Gag表达，尤其是在平滑肌细胞和结缔组织中。在1日龄雏鸡感染后，与产生病毒中和抗体的白来航鸡相比，在大多发展为耐受性病毒血症感染的21品系雏鸡中观察到更高的组织表达。源自HPRS - 103诱导的髓细胞性白血病的急性转化病毒966株显示出与HPRS - 103相似的嗜性。与A亚群禽白血病病毒RAV - 1株相比，HPRS - 103株在法氏囊淋巴滤泡髓质区域复制的倾向较低。这种低法氏囊嗜性可能是HPRS - 103不诱导淋巴细胞白血病的一个因素。HPRS - 103和966病毒在六个品系鸡的血液单核细胞培养物中复制，表明对髓单核细胞谱系有嗜性。相比之下，如先前报道，RAV - 1在单核细胞培养物中复制不佳，而B亚群禽白血病病毒RAV - 2则能复制。HPRS - 103对单核细胞的嗜性可能与其引起髓细胞性白血病的能力有关。六个品系的单核细胞和骨髓细胞培养物对966病毒转化的易感性差异排名相似，并显示出它们的相对易感性与这些品系的鸡对HPRS - 103诱导髓细胞性白血病的易感性相关的证据，表明这两种病毒在肿瘤发生过程中涉及共同的组织特异性病毒和宿主因素。
