短尾蝮蛇毒纤溶酶

Fibrinolytic enzyme from Agkistrodon halys brevicaudus (Korean mamushi) snake venom.

作者信息

Qian X H, Ma L

机构信息

Institute of Radiation Medicine, Academy of Medicine Science, Beijing, People's Republic of China.

出版信息

Toxicon. 1991;29(11):1381-6. doi: 10.1016/0041-0101(91)90125-b.

DOI:10.1016/0041-0101(91)90125-b

PMID:1814014

Abstract

By means of DEAE-Sepharose CL-6B ion exchange chromatography and TSK-GEL G2000 SW high-performance gel filtration, a purified protein with fibrinolytic activity was obtained from the venom of Agkistrodon halys brevicaudus (Korean mamushi). The protein was homogeneous as judged by isoelectric focusing electrophoresis and high-performance gel filtration. Its mol. wt is 39,200 and its isoelectric point 4.12. The specific fibrinolytic activity of the protein was 3.2 times higher than that of the crude venom. The fibrinolytic activity of the purified principle was 33 units/mg protein (units of standard urokinase activity).

摘要

通过DEAE-琼脂糖凝胶CL-6B离子交换色谱法和TSK-GEL G2000 SW高效凝胶过滤法，从短尾蝮蛇（朝鲜蝮蛇）毒液中获得了一种具有纤溶活性的纯化蛋白。通过等电聚焦电泳和高效凝胶过滤判断，该蛋白是均一的。其分子量为39,200，等电点为4.12。该蛋白的比纤溶活性比粗毒液高3.2倍。纯化产物的纤溶活性为33单位/毫克蛋白（标准尿激酶活性单位）。