一种用于从血斑中测定新生儿促甲状腺激素的快速荧光酶免疫测定法。

A rapid fluorometric enzyme immunoassay for the determination of neonatal TSH from blood spots.

作者信息

Tuuminen T, Rakkolainen A E, Welin M G, Weber T H, Nylander P L, Käpyaho K I

机构信息

Labsystems Oy, Helsinki, Finland.

出版信息

Clin Chim Acta. 1991 Oct 31;202(3):167-77. doi: 10.1016/0009-8981(91)90047-g.

DOI:10.1016/0009-8981(91)90047-g

PMID:1814645

Abstract

We describe a novel method for the detection of thyrotropin from dried blood spots using a horseradish peroxidase-labelled sandwich enzyme immunoassay with fluorometric detection. The detection limit of the present assay is 1.25 mIU/l with within-run and between-run imprecision being in the range 5.2 to 11.4%. The results of the assay correlate well with two commercial methods: an enzyme immunoassay (r = 0.93) and a time-resolved fluorescence assay (r = 0.90). The blood spot values also show a good correlation (r = 0.93) with respective values obtained from plasma using a commercial immunoradiometric method. The assay may also be performed colorimetrically with sensitivity similar to the fluorometric assay. However, the latter provides a wider dynamic range with an upper limit of 400 mIU/l while the colorimetric method reaches a plateau at 25 mIU/l. Due to its simplicity and rapid performance (3 h), the fluorometric assay is suitable for the routine screening of congenital hypothyroidism.

摘要

我们描述了一种使用辣根过氧化物酶标记的夹心酶免疫测定法并结合荧光检测从干血斑中检测促甲状腺激素的新方法。本测定法的检测限为1.25 mIU/l，批内和批间不精密度在5.2%至11.4%范围内。该测定法的结果与两种商业方法相关性良好：酶免疫测定法（r = 0.93）和时间分辨荧光测定法（r = 0.90）。血斑值与使用商业免疫放射测定法从血浆中获得的相应值也显示出良好的相关性（r = 0.93）。该测定法也可以比色法进行，其灵敏度与荧光测定法相似。然而，后者提供了更宽的动态范围，上限为400 mIU/l，而比色法在25 mIU/l时达到平台期。由于其简单性和快速性（3小时），荧光测定法适用于先天性甲状腺功能减退症的常规筛查。