Thuy L P, Sweetman L, Nyhan W L
Department of Pediatrics, La Jolla, CA 92093.
Clin Chim Acta. 1991 Oct 31;202(3):191-7. doi: 10.1016/0009-8981(91)90049-i.
A double antibody technique has been developed to separate free biotin from bound biotin after competitive binding of [3H]biotin and unlabelled biotin to avidin. Antiavidin goat antibody was added followed by the addition of antigoat IgG antibody linked to agarose. Centrifugation separated the free biotin from the biotin bound to the avidin complex. The method was suitable for the detection of the amounts of biotin contained in 100-200 microliters of plasma or 5-10 microliters of urine. Normal values for the concentration of biotin in plasma and urine determined by this assay were 1.27 +/- 0.67 nmol/l and 49.1 +/- 35.7 mumol/mol creatinine, respectively.
已开发出一种双抗体技术，用于在[3H]生物素和未标记的生物素与抗生物素蛋白竞争性结合后，将游离生物素与结合的生物素分离。加入抗抗生物素蛋白山羊抗体，随后加入与琼脂糖相连的抗山羊IgG抗体。离心将游离生物素与结合在抗生物素蛋白复合物上的生物素分离。该方法适用于检测100 - 200微升血浆或5 - 10微升尿液中所含生物素的量。通过该测定法确定的血浆和尿液中生物素浓度的正常值分别为1.27±0.67纳摩尔/升和49.1±35.7微摩尔/摩尔肌酐。
