Sanghvi R S, Lemons R M, Baker H, Thoene J G
Clin Chim Acta. 1982 Sep 1;124(1):85-90. doi: 10.1016/0009-8981(82)90322-9.
Measurement of biotin in plasma and urine has been stimulated by recent descriptions of inborn errors of biotin metabolism and by newly recognized causes of biotin deficiency. Biotin determination in physiologic fluids to document these conditions has been hindered by lack of a widely useable assay. This paper presents a method which employs tritium-labelled biotin, avidin, and nitrocellulose filters to measure urinary and plasma biotin in a rapid and simple manner.
近期对生物素代谢先天性缺陷的描述以及新发现的生物素缺乏原因，激发了对血浆和尿液中生物素的测量。由于缺乏广泛可用的检测方法，通过测定生理体液中的生物素来记录这些情况受到了阻碍。本文介绍了一种方法，该方法使用氚标记的生物素、抗生物素蛋白和硝酸纤维素滤膜，以快速简便的方式测量尿液和血浆中的生物素。
