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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Veldhuizen-Tsoerkan M B, van der Mast C A, Holwerda D A

Department of Experimental Zoology, University of Utrecht, The Netherlands.

Comp Biochem Physiol B. 1991;100(3):475-80. doi: 10.1016/0305-0491(91)90207-t.

Sea mussels were exposed to cadmium for short periods of time. The excised gills were incubated with radioactive orthophosphate. The gill proteins were separated by 1- and 2-dimensional gel electrophoresis and the phosphorylation state of the proteins was determined by image analysis of autoradiographs. 2. 1-Dimensional gel electrophoresis revealed that exposure of the animals to cadmium stimulated phosphorylation of the gill proteins in a cadmium concentration-dependent manner. 3. 2-Dimensional gel electrophoresis showed that cadmium differentially affected the phosphorylation of various proteins. Major alterations were observed in the basic, high mol. wt proteins and in the acidic, low mol. wt polypeptides.

将贻贝短时间暴露于镉中。切除鳃后，用放射性正磷酸盐进行孵育。通过一维和二维凝胶电泳分离鳃蛋白，并通过放射自显影片的图像分析确定蛋白的磷酸化状态。2. 一维凝胶电泳显示，动物暴露于镉中会以镉浓度依赖的方式刺激鳃蛋白的磷酸化。3. 二维凝胶电泳表明，镉对各种蛋白的磷酸化有不同影响。在碱性、高分子量蛋白和酸性、低分子量多肽中观察到主要变化。

Veldhuizen-Tsoerkan M B, van der Mast C A, Holwerda D A

Department of Experimental Zoology, University of Utrecht, The Netherlands.

Comp Biochem Physiol B. 1991;100(3):475-80. doi: 10.1016/0305-0491(91)90207-t.

Sea mussels were exposed to cadmium for short periods of time. The excised gills were incubated with radioactive orthophosphate. The gill proteins were separated by 1- and 2-dimensional gel electrophoresis and the phosphorylation state of the proteins was determined by image analysis of autoradiographs. 2. 1-Dimensional gel electrophoresis revealed that exposure of the animals to cadmium stimulated phosphorylation of the gill proteins in a cadmium concentration-dependent manner. 3. 2-Dimensional gel electrophoresis showed that cadmium differentially affected the phosphorylation of various proteins. Major alterations were observed in the basic, high mol. wt proteins and in the acidic, low mol. wt polypeptides.

将贻贝短时间暴露于镉中。切除鳃后，用放射性正磷酸盐进行孵育。通过一维和二维凝胶电泳分离鳃蛋白，并通过放射自显影片的图像分析确定蛋白的磷酸化状态。2. 一维凝胶电泳显示，动物暴露于镉中会以镉浓度依赖的方式刺激鳃蛋白的磷酸化。3. 二维凝胶电泳表明，镉对各种蛋白的磷酸化有不同影响。在碱性、高分子量蛋白和酸性、低分子量多肽中观察到主要变化。