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长期暴露于镉后，贻贝（紫贻贝）鳃组织在正常和热休克条件下应激蛋白的合成

Synthesis of stress proteins under normal and heat shock conditions in gill tissue of sea mussels (Mytilus edulis) after chronic exposure to cadmium.

作者信息

Veldhuizen-Tsoerkan M B, Holwerda D A, van der Mast C A, Zandee D I

机构信息

Department of Experimental Zoology, University of Utrecht, The Netherlands.

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1991;100(3):699-706. doi: 10.1016/0742-8413(91)90063-y.

DOI:10.1016/0742-8413(91)90063-y
PMID:1687571
Abstract
  1. Sea mussels were exposed to 16.5 micrograms Cd/1 under semi-field conditions for almost one year. The isolated gills were incubated with 35S-methionine or -cysteine. 2. Chronic exposure to cadmium neither altered the rate of amino acid incorporation nor induced expression of heat shock proteins in the gills. 3. Heat shock imposed after chronic exposure to cadmium resulted in an increased synthesis of heat shock proteins, especially those of high molecular weight. 4. Synthesis of cadmium-binding, low molecular weight proteins was observed at any point of the exposure time. Their cadmium-binding capacity and rate of synthesis, after the initial increase, remained unchanged throughout the exposure.
摘要

  1. 在半野外条件下，将贻贝暴露于16.5微克镉/升的环境中近一年。分离出的鳃组织用35S-甲硫氨酸或35S-半胱氨酸进行孵育。

  2. 长期接触镉既未改变鳃组织中氨基酸的掺入率，也未诱导热休克蛋白的表达。

  3. 在长期接触镉后施加热休克，导致热休克蛋白的合成增加，尤其是高分子量的热休克蛋白。

  4. 在暴露期间的任何时间点都观察到了镉结合低分子量蛋白的合成。它们的镉结合能力和合成速率在最初增加后，在整个暴露过程中保持不变。

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1
Synthesis of stress proteins under normal and heat shock conditions in gill tissue of sea mussels (Mytilus edulis) after chronic exposure to cadmium.长期暴露于镉后，贻贝（紫贻贝）鳃组织在正常和热休克条件下应激蛋白的合成
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1991;100(3):699-706. doi: 10.1016/0742-8413(91)90063-y.
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Toxicol Appl Pharmacol. 1994 Apr;125(2):206-13. doi: 10.1006/taap.1994.1066.
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Accumulation of cadmium and lead in the gills of Mytilus edulis: X-ray microanalysis and chemical analysis.
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[Changes of heat shock proteins content in the gill epithelium cells of mussel Mytilus edulis L. depending upon the salinity of medium].[贻贝鳃上皮细胞中热休克蛋白含量随培养基盐度的变化]
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