Miao T J, Wang Z, Sang N
Department of Urology, Zhongshan Hospital, Shanghai Medical University, PRC.
Eur Urol. 1991;20(4):307-10.
It has been proven that the abnormal activation of ras oncogene and the expression of its product P21, a 21,000-dalton molecular weight protein, are present in a number of human tumors. Using an ABC immunoencymatic staining technique and a P21-specific monoclonal antibody, SCI-Oncogema I, we have detected the expression of P21 in formalin-fixed, paraffin-embedded samples from 53 bladder tumors and 7 normal bladder mucosae. The chi 2 test was used for data analysis. The results demonstrate that P21 is only expressed on tumor tissues. It is also seen that the percentage of P21 positivity increases, obviously in parallel with the increase of pathological grade and stage. The morbidity rate due to tumor recurrence in 32 P21-positive patients was only 9% (3/32), whereas that in 21 P21-negative cases was 67% (14/21). This suggests that in patients with bladder tumors P21 positivity could be considered as an objective indicator of a favorable prognosis.
业已证实，ras癌基因的异常激活及其产物P21（一种分子量为21,000道尔顿的蛋白质）的表达存在于多种人类肿瘤中。我们使用ABC免疫酶染色技术和一种P21特异性单克隆抗体SCI-Oncogema I，检测了53例膀胱肿瘤和7例正常膀胱黏膜的福尔马林固定、石蜡包埋样本中P21的表达。采用卡方检验进行数据分析。结果表明，P21仅在肿瘤组织中表达。还可以看出，P21阳性率明显随着病理分级和分期的增加而升高。32例P21阳性患者的肿瘤复发率仅为9%（3/32），而21例P21阴性患者的肿瘤复发率为67%（14/21）。这表明，在膀胱肿瘤患者中，P21阳性可被视为预后良好的客观指标。
