Salinas P, Barrigón S
Department of Pharmacology, School of Medicine, Complutense University, Madrid, Spain.
Eur J Pharmacol. 1991 Dec 10;209(1-2):105-7. doi: 10.1016/0014-2999(91)90018-l.
The ouabain threshold to induce cardiac arrhythmias in urethane-anaesthetized guinea-pigs was not modified by the administration of either dazmegrel, 4 mg/kg i.v., or lysine-acetylsalicylate, 13.5 mg/kg i.v., 5 min before the infusion of ouabain,10g/kg per min i.v. The previous administration of platelet-activating factor (PAF), 0.01 to 1 nmol/animal i.v., 2 min prior to ouabain, caused a significant, dose-dependent decrease of the ouabain threshold for the cardiac rhythm disturbances. Lysine-acetylsalicylate lacked any effect on this PAF potentiation. Pretreatment with dazmegrel 5 min before PAF, 0.05 nmol/animal i.v., abolished the PAF potentiation of the digitalis-induced arrhythmias. These results suggest that thromboxane synthesis is involved in this PAF effect and indicate an ability of PAF to induce thromboxane generation even in the case of cyclooxygenase inhibition.
在静脉注射哇巴因（10g/kg每分钟）前5分钟，静脉注射4mg/kg的达美格雷或13.5mg/kg的赖氨酸乙酰水杨酸，对乌拉坦麻醉的豚鼠诱发心律失常的哇巴因阈值没有影响。在静脉注射哇巴因前2分钟，静脉注射0.01至1nmol/动物的血小板活化因子（PAF），会导致心律失常的哇巴因阈值显著降低，且呈剂量依赖性。赖氨酸乙酰水杨酸对这种PAF增强作用没有任何影响。在静脉注射0.05nmol/动物的PAF前5分钟用达美格雷预处理，可消除PAF对洋地黄诱导的心律失常的增强作用。这些结果表明，血栓素的合成参与了PAF的这种作用，并表明即使在环氧化酶受到抑制的情况下，PAF也有诱导血栓素生成的能力。
