培养的啮齿动物星形胶质细胞对去甲肾上腺素及其氧化产物的积累。
Accumulation of noradrenaline and its oxidation products by cultured rodent astrocytes.
作者信息
Wilson J X, Wilson G A
机构信息
Department of Physiology, University of Western Ontario, London, Canada.
出版信息
Neurochem Res. 1991 Nov;16(11):1199-205. doi: 10.1007/BF00966696.
The accumulation of [3H]noradrenaline ([3H]NA) and its oxidation products was studied in primary cultures of cerebral astrocytes. Astroglial accumulation of radiolabeled catecholamine ([3H] NA and oxidation products) was enhanced by manganese or iron, but it was inhibited by unlabeled NA, dopamine or ascorbate. Tissue: medium ratios of radioactivity increased as extracellular [3H]NA was oxidized. When extracellular oxidation was prevented by ascorbate, as confirmed by high performance liquid chromatography with electrochemical detection, either ouabain pretreatment or nominally Na(+)-free incubation medium inhibited approximately one-half of specific [3H]NA accumulation by rat (but not mouse) astrocytes. These observations suggest that neurological responses to trace metals and ascorbate may arise from the effects of these agents on the clearance of extracellular catecholamines. Astrocytes can accumulate oxidation products of NA more rapidly than they take up NA itself, but ascorbate at physiological concentrations prevents the oxidation process in extracellular fluid. Furthermore, in the presence of ascorbate, Na(+)-dependent transport mediates a significant component of NA accumulation in rat astrocytes.
在大脑星形胶质细胞的原代培养物中研究了[3H]去甲肾上腺素（[3H]NA）及其氧化产物的积累情况。锰或铁可增强放射性标记儿茶酚胺（[3H]NA和氧化产物）在星形胶质细胞中的积累，但未标记的NA、多巴胺或抗坏血酸会抑制这种积累。随着细胞外[3H]NA被氧化，组织与培养基的放射性比值增加。当通过高效液相色谱电化学检测证实抗坏血酸可防止细胞外氧化时，哇巴因预处理或名义上无Na(+)的孵育培养基可抑制大鼠（而非小鼠）星形胶质细胞特异性[3H]NA积累的约一半。这些观察结果表明，对痕量金属和抗坏血酸的神经学反应可能源于这些物质对细胞外儿茶酚胺清除的影响。星形胶质细胞积累NA氧化产物的速度比摄取NA本身更快，但生理浓度的抗坏血酸可防止细胞外液中的氧化过程。此外，在抗坏血酸存在的情况下，Na(+)依赖性转运介导了大鼠星形胶质细胞中NA积累的重要部分。
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