Hansson E
Neurochem Res. 1985 May;10(5):667-75. doi: 10.1007/BF00964405.
The transport of [3H]L-glutamate, [3H]L-aspartate, [3H] gamma-aminobutyric acid [( 3H]GABA), [3H]dopamine, [3H]norepinephrine and [3H]5-hydroxytryptamine (3H-5-HT) was measured in primary astroglial cultures from newborn rat cerebral hemispheres. There was a high-affinity uptake with a Km of 69.0 microM for L-glutamate, 12.3 microM for L-aspartate and 3.1 microM for GABA. The uptake showed properties of high capacity with a Vmax of 17.0 nmol X mg prot-1 X min-1 for L-glutamate, 1.1 nmol X mg prot-1 X min-1 for L-aspartate and 0.04 nmol X mg prot-1 X min-1 for GABA. No high-affinity high capacity transport system was found for the monoamines studied. Autoradiographic examination demonstrated a heavy deposit of grains suggesting a prominent accumulation of [3H]L-glutamate and [3H]L-aspartate in the astroglial-like cells of the cultures, while the [3H]GABA accumulation was less intense. On the other hand, there was only a weak accumulation of grains after incubating the cultures with [3H]dopamine, [3H]norepinephrine or [3H]5-HT. Thus, astroglial cells in culture accumulate amino acid neurotransmitters and monoamines in different ways with a high-affinity high-capacity uptake of glutamate, aspartate and GABA and a diffusion-uptake of dopamine, norepinephrine and 5-HT.
在新生大鼠脑半球的原代星形胶质细胞培养物中，对[³H]L-谷氨酸、[³H]L-天冬氨酸、[³H]γ-氨基丁酸[（³H）GABA]、[³H]多巴胺、[³H]去甲肾上腺素和[³H]5-羟色胺（³H-5-HT）的转运进行了测定。L-谷氨酸的摄取具有高亲和力，其Km为69.0微摩尔，L-天冬氨酸为12.3微摩尔，GABA为3.1微摩尔。摄取表现出高容量特性，L-谷氨酸的Vmax为17.0纳摩尔×毫克蛋白⁻¹×分钟⁻¹，L-天冬氨酸为1.1纳摩尔×毫克蛋白⁻¹×分钟⁻¹，GABA为0.04纳摩尔×毫克蛋白⁻¹×分钟⁻¹。在所研究的单胺中未发现高亲和力高容量转运系统。放射自显影检查显示有大量银粒沉积，表明培养物中类星形胶质细胞显著积累了[³H]L-谷氨酸和[³H]L-天冬氨酸，而[³H]GABA的积累则较弱。另一方面，用[³H]多巴胺、[³H]去甲肾上腺素或[³H]5-HT孵育培养物后，银粒积累很弱。因此，培养的星形胶质细胞以不同方式积累氨基酸神经递质和单胺，对谷氨酸、天冬氨酸和GABA具有高亲和力高容量摄取，对多巴胺、去甲肾上腺素和5-HT则是扩散摄取。
