Thomas C E
Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Santa Fe, Argentina.
Rev Argent Microbiol. 1991 Jul-Sep;23(3):123-9.
The contact between polymyxin B and human blood cells produced a double effect on the haemolytic activity of Listeria monocytogenes, L. seeligeri and L. ivanovii hemolysins. At low polymyxin B concentrations the antibiotic promoted erythrocyte lysis whereas at higher antibiotic levels, hemolysis inhibition was observed. According to the effects of polymyxin B, hemolytic profiles were established for each hemolysin. L. monocytogenes and L. seeligeri exhibited a similar profile with maximum hemolytic activity at 1000 U/ml polymyxin B. L. ivanovii displayed a different profile, with two maximum hemolytic activities: one similar to that showed by L. monocytogenes and L. seeligeri at 1000 U/ml, and another peak of hemolysis at 4000 U/ml polymyxin B.
多粘菌素B与人类血细胞的接触对单核细胞增生李斯特菌、斯氏李斯特菌和伊氏李斯特菌溶血素的溶血活性产生了双重影响。在低浓度多粘菌素B时，抗生素促进红细胞裂解，而在抗生素水平较高时，则观察到溶血抑制。根据多粘菌素B的作用，为每种溶血素建立了溶血谱。单核细胞增生李斯特菌和斯氏李斯特菌表现出相似的谱，在1000 U/ml多粘菌素B时溶血活性最高。伊氏李斯特菌表现出不同的谱，有两个最大溶血活性：一个与单核细胞增生李斯特菌和斯氏李斯特菌在1000 U/ml时的相似，另一个溶血峰值在4000 U/ml多粘菌素B时出现。
