Dominguez Rodriguez L, Vazquez Boland J A, Fernandez Garayzabal J F, Echalecu Tranchant P, Gomez-Lucia E, Rodriguez Ferri E F, Suarez Fernandez G
J Clin Microbiol. 1986 Jul;24(1):99-103. doi: 10.1128/jcm.24.1.99-103.1986.
Because the hemolysis produced by Listeria monocytogenes and Listeria seeligeri on blood agar is frequently difficult to interpret, we developed a microplate technique for the routine determination of hemolytic activity with erythrocyte suspensions. This microtechnique is a simple and reliable test for distinguishing clearly between hemolytic and nonhemolytic strains and could be used instead of the CAMP (Christie-Atkins-Munch-Petersen) test with Staphylococcus aureus in the routine typing of Listeria strains. Furthermore, our results suggest that the quantitation of the hemolytic activity of the Listeria strains, along with the D-xylose, L-rhamnose, and alpha-methyl-D-mannoside acidification tests, allows the differentiation of L. monocytogenes, L. seeligeri, and Listeria ivanovii. We also observed that the treatment of erythrocytes with crude exosubstances of rhodococcus equi, Pseudomonas fluorescens, Acinetobacter calcoaceticus, and S. aureus enhanced the hemolytic activity of all Listeria strains with this characteristic.
由于单核细胞增生李斯特菌和斯氏李斯特菌在血琼脂上产生的溶血现象常常难以判断，我们开发了一种微孔板技术，用于用红细胞悬液常规测定溶血活性。这种微量技术是一种简单可靠的测试方法，能清晰区分溶血菌株和非溶血菌株，在李斯特菌菌株的常规分型中可替代与金黄色葡萄球菌进行的CAMP（克里斯蒂-阿特金斯-芒奇-彼得森）试验。此外，我们的结果表明，对李斯特菌菌株溶血活性进行定量，再结合D-木糖、L-鼠李糖和α-甲基-D-甘露糖苷酸化试验，可区分单核细胞增生李斯特菌、斯氏李斯特菌和伊氏李斯特菌。我们还观察到，用马红球菌、荧光假单胞菌、醋酸钙不动杆菌和金黄色葡萄球菌的粗胞外物质处理红细胞，可增强所有具有此特征的李斯特菌菌株的溶血活性。
