Fang Bin, Liu Hongying, Wang Guangfeng, Zhou Yunyou, Li Maoguo, Yu Yan, Zhang Wei
School of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Ann Chim. 2007 Oct;97(10):1005-13. doi: 10.1002/adic.200790085.
9-Aminoacridine was firstly immobilized on the surface of a glassy carbon electrode to form a poly (9-aminoacridine) film modified electrode. The results demonstrated that the modified electrode exhibited a high degree of catalytic activity towards the oxidation of tyrosine and can resolve the interference of tryptophan in the determination of tyrosine. Compared with the bare electrode, the peak current had obviously increased, and the peak potential had shifted in a negative direction. Under the optimum conditions, a linear response to tyrosine was observed in the concentration of 1.0 x 10(-6) -2.8 x 10(-3) M, with a correlation coefficient of 0.9987, and a detection limit (S/N = 3) of 1.0 x 10(-7) M. The modified electrode has been successfully applied to determine the concentration of tyrosine in composite amino acid injections; and it displays excellent repeatability and high sensitivity. The proposed sensor has promising features such as ease of fabrication, good reproducibility, high stability and low cost. And most of all, it has good selectivity.
首先将9-氨基吖啶固定在玻碳电极表面，形成聚（9-氨基吖啶）膜修饰电极。结果表明，修饰电极对酪氨酸的氧化表现出高度的催化活性，并且在酪氨酸测定中能够消除色氨酸的干扰。与裸电极相比，峰电流明显增加，峰电位向负方向移动。在最佳条件下，酪氨酸浓度在1.0×10⁻⁶ - 2.8×10⁻³ M范围内呈线性响应，相关系数为0.9987，检测限（S/N = 3）为1.0×10⁻⁷ M。该修饰电极已成功应用于复合氨基酸注射液中酪氨酸浓度的测定；并且显示出优异的重复性和高灵敏度。所提出的传感器具有易于制备、良好的重现性、高稳定性和低成本等有前景的特点。最重要的是，它具有良好的选择性。
