Miyake Noriko, Miyake Kazunori, Higurashi Hitomi, Ohsaka Akimichi
Department of Clinical Laboratory Medicine, Juntendo University, School of Medicine, Bunkyo-ku, Tokyo.
Rinsho Byori. 2007 Nov;55(11):1019-24.
Serum lipid profiles have usually been evaluated using blood specimen in fasting state. However, postprandial hypertriglyceridemia is important risk factor for atherosclerosis. In the present study, we determined several parameters of triglyceride (TG) metabolism in healthy volunteers. Serum concentrations of TG in fasting state correlated negative the ratio of preheparin serum lipoprotein lipase mass (pLPL) and apoC-III (pLPL/apoC-III) in both fasting (r = -0.771, p < 0.0001) and postprandial (r = -0.640, p < 0.0001) state. To exclude the effect of high density lipoprotein (HDL) in serum, we purified the fraction of TG rich lipoprotein (TRL) using ultracentrifugation method 3 healthy volunteers with postprandial state. The pLPL/apoC-III was reduced constantly during postprandial state in all volunteers. These findings suggest that pLPL/apoC-III may be a useful marker for evaluation of TG metabolism using postprandial blood specimens.
血清脂质谱通常使用空腹状态下的血液样本进行评估。然而，餐后高甘油三酯血症是动脉粥样硬化的重要危险因素。在本研究中，我们测定了健康志愿者甘油三酯（TG）代谢的几个参数。空腹状态下血清TG浓度与空腹（r = -0.771，p < 0.0001）和餐后（r = -0.640，p < 0.0001）状态下肝素前血清脂蛋白脂肪酶质量（pLPL）与载脂蛋白C-III（pLPL/apoC-III）的比值呈负相关。为排除血清中高密度脂蛋白（HDL）的影响，我们采用超速离心法对3名处于餐后状态的健康志愿者的富含甘油三酯脂蛋白（TRL）部分进行了纯化。所有志愿者在餐后状态下pLPL/apoC-III均持续降低。这些发现表明，pLPL/apoC-III可能是使用餐后血液样本评估TG代谢的有用标志物。
