Vafin R R, Ravilov R Kh, Gaffarov Kh Z, Ravilov A Z, Iskhakov G M, Bakirov I Kh, Kashov V N, Vafin R R
Mol Gen Mikrobiol Virusol. 2007(4):17-25.
The results of molecular-genetic study of typical specimens of the genus Chlamydophila of the family Chlamydiaceae from the FSE FCTRSA collection of microorganisms are considered for the purpose of their taxonomy based on comparative analysis by the omp1-, omp2-, 16S rRNA-, and 23S rRNA-genes and by the presence of extrachromosomal plasmid with corresponding fragments of the genomes officially registered species of chlamydiae. According to the omp1-RFLP-AluI-profile, the Rostinovo-70, 250, PP-87, and KC-93 strains were characterized by new genotype of chlamydiae (genotype G). The new omp2-RFLP-AluI-profile of chlamydiae on carrying out the comparative analysis of Rostinovo-70, 250, PP-87 and KC-93 strains by omp2-gene was characterized. On the basis of the species identification of chlamydiae by genetical and ecological criteria, the chlamydia strains Rostinovo-70, 250, PP-87, and KC-93 can be included in the new chlamydia species Chlamydophila parapsittaci sp. nov. It was suggested to combine into a new species the following strains of Chlamydophila psittaci: WC, NJ1, 92-1293, TT3, 7344/2, GD, CT1, Par1, 84/2334, R54, VS225, 777B15, and Prk/Daruma.
为了基于omp1、omp2、16S rRNA和23S rRNA基因以及通过衣原体官方注册物种基因组相应片段的染色体外质粒的存在进行比较分析来对衣原体科嗜衣原体属的典型标本进行分类，对来自俄罗斯联邦国家研究中心联邦科学中心微生物保藏库（FSE FCTRSA）的微生物进行了分子遗传学研究。根据omp1-RFLP-AluI图谱，罗斯蒂诺沃-70、250、PP-87和KC-93菌株具有衣原体新基因型（基因型G）。通过omp2基因对罗斯蒂诺沃-70、250、PP-87和KC-93菌株进行比较分析时，确定了衣原体新的omp2-RFLP-AluI图谱。基于遗传学和生态学标准对衣原体进行物种鉴定，罗斯蒂诺沃-70、250、PP-87和KC-93衣原体菌株可归入新的鹦鹉热嗜衣原体物种。建议将以下鹦鹉热嗜衣原体菌株合并为一个新物种：WC、NJ1、92-1293、TT3、7344/2、GD、CT1、Par1、84/2334、R54、VS225、777B15和Prk/Daruma。
