Tesana Smarn, Srisawangwong Tuanchai, Sithithaworn Paiboon, Laha Thewarach
Food-Borne Parasite Research Group, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Exp Parasitol. 2008 Apr;118(4):531-5. doi: 10.1016/j.exppara.2007.11.007. Epub 2007 Nov 19.
Six groups (15 snails/group) of Pomacea canaliculata and Pila polita were infected orally with 0 (control), 200, 400, 800, 1600 and 3200 first-stage Angiostrongylus cantonensis larvae (L1). The respective mean+/-SD third stage larvae (L3) worm recovery 1-month post-infection (p.i.) for P. canaliculata was 0, 1.4+/-5.42 (0.7%), 0.13+/-0.35 (0.03%), 0.07+/-0.26 (0.009%), 0.07+/-0.26 (0.004%), 0, and for P. polita 0, 64.33+/-21.38 (32.25%), 115.36+/-36.82 (28.93%), 265.33+/-90.01 (33.27%), 471.33+/-92.98 (29.60%) and 849.00+/-243.23 (26.61%). The susceptibility of A. cantonensis in P. polita was dose-dependent (p<0.001). In the three groups (nine snails/group) of P. polita given 500 L1, we studied the distribution of L3 in the internal organs (i.e., foot, head+esophagus, kidney, albumin gland, mantle, intestine, digestive gland) and found the highest density after 1, 2 and 3 months p.i. in the mantle at 29.37%, 31.09% and 37.45%. The infection rate in P. canaliculata was too low to study distribution rates.
将六组(每组15只蜗牛)福寿螺和梨形环棱螺经口感染0(对照组)、200、400、800、1600和3200条广州管圆线虫一期幼虫(L1)。感染后1个月(p.i.),福寿螺相应的平均±标准差三期幼虫(L3)回收率分别为0、1.4±5.42(0.7%)、0.13±0.35(0.03%)、0.07±0.26(0.009%)、0.07±0.26(0.004%)、0;梨形环棱螺相应的平均±标准差三期幼虫(L3)回收率分别为0、64.33±21.38(32.25%)、115.36±36.82(28.93%)、265.33±90.01(33.27%)、471.33±92.98(29.60%)和849.00±243.23(26.61%)。梨形环棱螺对广州管圆线虫的易感性呈剂量依赖性(p<0.001)。在三组(每组9只蜗牛)感染500条L1的梨形环棱螺中,我们研究了三期幼虫(L3)在内部器官(即足部、头部+食管、肾脏、蛋白腺、外套膜、肠道、消化腺)中的分布,发现在感染后1、2和3个月,外套膜中的密度最高,分别为29.37%、31.09%和37.45%。福寿螺的感染率过低,无法研究分布率。