Wang Jian-Ming, Xu Jin-Jun, Zhou Yong-Hua, Tao Jian-Ping
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2014 Dec;26(6):675-7.
To observe the effect of artificially construction of the life cycle of Angiostrongylus cantonensis in the laboratory condition, so as to provide the basis for the research of angiostrongyliasis.
SD rats were infected orally with the third-stage larvae of A. cantonensis collected from infected Pomacea canaliculata. Six weeks after the infection, the first-stage larvae were isolated and counted from fresh feces of the rats and then were used to infect P. canaliculata. Three weeks later, the snails were dissected for counting the third-staged larvae of A. cantonensis.
The first-stage larvae were detected in the feces of the rats 6 weeks after the infection, and the third-staged larvae were successfully isolated after the infection of P. canaliculata.
The animal model of the entire life cycle of A. cantonensis is successfully established in the laboratory with the infection of 50 larvae per rat.
观察在实验室条件下人工构建广州管圆线虫生命周期的效果，为广州管圆线虫病的研究提供依据。
将从感染福寿螺中收集的广州管圆线虫第三期幼虫经口感染SD大鼠。感染6周后，从大鼠新鲜粪便中分离并计数第一期幼虫，然后用于感染福寿螺。3周后，解剖福寿螺以计数广州管圆线虫第三期幼虫。
感染6周后在大鼠粪便中检测到第一期幼虫，感染福寿螺后成功分离出第三期幼虫。
以每只大鼠感染50条幼虫，在实验室成功建立了广州管圆线虫完整生命周期的动物模型。
