Zhang Chao-Wei, Zhou Xiao-Nong, Lv Shan, Zhang Yi, Liu He-Xiang
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2008 Jun 30;26(3):203-4, 209.
To observe the morphologic characteristics of III stage larvae of Angiostrongylus cantonensis from Pomacea canaliculata.
P. canaliculata, the intermediate host snail of A. cantonensis, was infected with I stage larvae of A. cantonensis in laboratory. After 61 days, III stage larvae of A. cantonensis were harvested from snail's lungs and muscle of head-foot, followed by HE stain to observe morphological characteristics.
The whole body of III stage larva was curling with obtuse head. Its pharyngeal canal extends from the buccal hole on the top of the head to the intestines at the pharyngeal intestine joint place, with apex cauda and clear anal tube. The tegument of the III stage larva was eosin-stained, with a transparent sheath outside of tegument. Some of the larvae cauda showed in circular cylinder, and some larvae presented ventral gland with two very short uterine which used to be the feature only showed in early IV stage larva.
Morphologically characteristics of the III stage larvae is helpful to better understand the life-cycle and the control of A. cantonensis.
观察福寿螺体内广州管圆线虫Ⅲ期幼虫的形态特征。
在实验室将广州管圆线虫Ⅰ期幼虫感染福寿螺这一广州管圆线虫中间宿主螺。61天后，从螺的肺及头足部肌肉中采集广州管圆线虫Ⅲ期幼虫，进行苏木精-伊红（HE）染色以观察形态特征。
Ⅲ期幼虫全身卷曲，头部钝圆。咽管从头部顶端的口孔延伸至咽肠连接处的肠道，尾部尖，肛管清晰。Ⅲ期幼虫体表被伊红染色，体表外有一层透明鞘。部分幼虫尾部呈圆柱形，部分幼虫出现腹侧腺及两条很短的子宫，而这一特征以往仅在Ⅳ期早期幼虫中出现。
Ⅲ期幼虫的形态特征有助于更好地了解广州管圆线虫的生命周期及防治。
