Mojaat M, Foucault A, Pruvost J, Legrand J
Université de Nantes, CNRS, GEPEA UMR-CNRS 6144, Boulevard de l'Université, CRTT-BP 406, 44602 Saint-Nazaire Cedex, France.
J Biotechnol. 2008 Feb 29;133(4):433-41. doi: 10.1016/j.jbiotec.2007.11.003. Epub 2007 Nov 19.
In the aim of beta-carotene biocompatible extraction, toxicity of various pure solvents belonging to different homologous series has been investigated for Dunaliella salina. The results showed that solvents having logP(oct) > 5 or having a molecular weight over 150 g/mol can be considered biocompatible for this microalga. The membrane critical solvent concentration for each series of solvents has been calculated applying Osborne's model, showing that the aliphatic chlorinated hydrocarbon is the most toxic family studied. Mixtures of a biocompatible solvent (decane) with a toxic solvent (CH(2)Cl(2), MEK, MTBE) have been studied. The beta-carotene extraction ability of CH(2)Cl(2)-decane mixture was found six times more efficient than with pure decane. It has been demonstrated that the extraction ability of solvent depends on its affinity with the product extracted and on its concentration incorporated in the cellular membrane.
为了实现β-胡萝卜素的生物相容性提取，研究了不同同系物系列的各种纯溶剂对盐生杜氏藻的毒性。结果表明，logP（辛醇）>5或分子量超过150 g/mol的溶剂可被认为对这种微藻具有生物相容性。应用奥斯本模型计算了每个溶剂系列的膜临界溶剂浓度，结果表明脂肪族氯代烃是所研究的毒性最大的族。研究了生物相容性溶剂（癸烷）与有毒溶剂（CH₂Cl₂、MEK、MTBE）的混合物。发现CH₂Cl₂-癸烷混合物提取β-胡萝卜素的能力比纯癸烷高六倍。已经证明，溶剂的提取能力取决于其与被提取产物的亲和力以及其在细胞膜中的掺入浓度。
