Ederli Nicole Brand, Bonadiman Sérgio Fernandes, de Moraes Neto Antonio Henrique Almeida, DaMatta Renato Augusto, de Paula Santos Clóvis
Laboratório de Biologia Celular e Tecidual, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, 28013-602 Campos dos Goytacazes, RJ, Brazil.
Vet Parasitol. 2008 Feb 14;151(2-4):227-32. doi: 10.1016/j.vetpar.2007.11.009. Epub 2007 Nov 17.
The genus Libyostrongylus includes three species, L. douglassii, L. dentatus and L. magnus that occur as parasites in the proventriculus of Struthio camelus. We confirmed a mixed infection by L. douglassii and L. dentatus in farmed ostriches from the southeast of Brazil for the first time, and provided new information on some morphological characters that differentiate these species. Adult nematodes collected from the proventriculus of ostriches were observed by light and scanning electron microscopy. Morphologic characterization and morphometric analysis of the nematodes enabled the distinction of both species and corroborated results of prior studies. Specimens of L. dentatus have a buccal capsule with a prominent esophageal tooth. Furthermore, males and females of L. dentatus were larger (4954 and 9347 microm) than those of L. douglassii (3411 and 4229 microm), but measurements for most characters in both species were smaller then those previously reported. Besides, the cephalic structure based on scanning electron microscopy differs, and L. dentatus has thick lips with round papillae, whereas L. douglassii has fine lips with lengthened papillae. The confirmation of both species in South America strongly suggests that the mixed infection may be common in farmed ostriches.
利比亚圆线虫属包括三个物种，即道格拉斯利比亚圆线虫、有齿利比亚圆线虫和大型利比亚圆线虫，它们寄生于鸵鸟的前胃。我们首次在巴西东南部养殖鸵鸟中证实了道格拉斯利比亚圆线虫和有齿利比亚圆线虫的混合感染，并提供了一些区分这些物种的形态特征的新信息。通过光学显微镜和扫描电子显微镜观察了从鸵鸟前胃收集的成年线虫。线虫的形态特征和形态测量分析能够区分这两个物种，并证实了先前研究的结果。有齿利比亚圆线虫的标本有一个带有突出食管齿的口囊。此外，有齿利比亚圆线虫的雄性和雌性（分别为4954微米和9347微米）比道格拉斯利比亚圆线虫的（分别为3411微米和4229微米）更大，但两个物种大多数特征的测量值都比先前报道的小。此外，基于扫描电子显微镜的头部结构不同，有齿利比亚圆线虫有厚唇和圆形乳头，而道格拉斯利比亚圆线虫有细唇和拉长的乳头。在南美洲确认这两个物种强烈表明，混合感染在养殖鸵鸟中可能很常见。
