Ederli Nicole Brand, de Oliveira Francisco Carlos Rodrigues, Lopes Carlos Wilson Gomes, Damatta Renato Augusto, Santos Clóvis de Paula, de Azevedo Rodrigues Maria de Lurdes
Laboratório de Helmintologia, Departamento de Parasitologia Animal (DPA), Instituto de Veterinária (IV), Universidade Federal Rural do Rio de Janeiro (UFRRJ), 23890-000 Seropédica, RJ, Brazil.
Vet Parasitol. 2008 Aug 17;155(3-4):323-7. doi: 10.1016/j.vetpar.2008.04.023. Epub 2008 May 10.
The differentiation of the species of the Libyostrongylus genus is only possible with the obtainment of the adult parasites in the ostriches proventriculus and gizzard. The present work confirms that it is possible to differentiate the infective larvae of L. douglassii and L. dentatus allowing the differential diagnosis of these species by fecal culture. To show this, adult females from both species were collected from ten proventriculus from adult ostriches and separated by species. Both groups were macerated individually added to sterilized feces for standard fecal cultures. The infective larvae were recovered, identified, quantified and measured. All proventriculus analyzed were parasitized by Libyostrongylus spp. and a clear heterogeneous location for each species was observed. The infective larvae from the fecal cultures of macerated L. douglassii presented a mean total length of 874.3+/-33.80 microm, and a short sheath tail (29.5+/-4.11 microm) with acute termination. The infective larvae from the macerated L. dentatus presented mean total length of 856.0+/-43.63 microm, long sheath tail (61.2+/-9.52 microm) with filamentous termination. The mean measures of the tails of both species had a significant difference. The differentiation of the infective larvae of L. douglassii and L. dentatus by fecal cultures will facilitate the diagnosis of both species for further understanding the Libyostrongylus biology.
只有在鸵鸟前胃和砂囊中获取成年寄生虫后，才能区分利比亚圆线虫属的物种。本研究证实，通过粪便培养可以区分道格拉斯利比亚圆线虫和有齿利比亚圆线虫的感染性幼虫，从而对这些物种进行鉴别诊断。为了证明这一点，从成年鸵鸟的十个前胃中收集了这两个物种的成年雌性，并按物种进行了分离。将两组分别进行研磨，然后添加到经过消毒的粪便中进行标准粪便培养。回收、鉴定、量化并测量感染性幼虫。所有分析的前胃均被利比亚圆线虫属寄生，并且观察到每个物种都有明显的异质分布。研磨后的道格拉斯利比亚圆线虫粪便培养物中的感染性幼虫平均总长度为874.3±33.80微米，鞘尾短（29.5±4.11微米），末端尖锐。研磨后的有齿利比亚圆线虫的感染性幼虫平均总长度为856.0±43.63微米，鞘尾长（61.2±9.52微米），末端呈丝状。两个物种尾巴的平均尺寸存在显著差异。通过粪便培养区分道格拉斯利比亚圆线虫和有齿利比亚圆线虫的感染性幼虫，将有助于对这两个物种进行诊断，以便进一步了解利比亚圆线虫的生物学特性。
