用通用方法测定大肠杆菌O111 O抗原的糖基转移酶特异性
Determination of glycosyltransferase specificities for the Escherichia coli O111 O antigen by a generic approach.
作者信息
Stevenson Gordon, Dieckelmann Manuela, Reeves Peter R
机构信息
School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia.
出版信息
Appl Environ Microbiol. 2008 Feb;74(4):1294-8. doi: 10.1128/AEM.02660-07. Epub 2007 Dec 21.
Abstract
We describe a bacterial strain developed to facilitate the determination of glycosyltransferase (GT) specificities for O antigens of known structure and gene cluster sequence. For proof of principle for the approach, the strain was used to determine the specificity of the Escherichia coli O111 O-antigen GT genes.
摘要
我们描述了一种为便于确定已知结构和基因簇序列的O抗原的糖基转移酶（GT）特异性而构建的菌株。为了验证该方法的原理，使用该菌株来确定大肠杆菌O111 O抗原GT基因的特异性。
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