Kelley Darshan S, Siegel David, Vemuri Madhuri, Chung Gloria H, Mackey Bruce E
Western Human Nutrition Research Center, Agricultural Research Service, USDA and Department of Nutrition, University of California, Davis, CA 95616, USA.
J Nutr. 2008 Jan;138(1):30-5. doi: 10.1093/jn/138.1.30.
Plasma remnant-like particle-cholesterol (RLP-C) and the RBC (n-3) index are novel risk factors for cardiovascular disease. Effects of docosahexaenoic acid (DHA) supplementation on these risk factors in hypertriglyceridemic men have not been studied. We determined effects of DHA supplementation on concentrations of plasma RLP-C, the RBC (n-3) index, and associations between concentrations of plasma RLP-C with those of plasma lipids and fatty acids. Hypertriglyceridemic men aged 39-66 y, participated in a randomized, placebo-controlled, parallel study. They received no supplements for 8 d and then received either 7.5 g/d DHA oil (3 g DHA/d) or olive oil (placebo) for the last 90 d. Fasting blood samples were collected on study d -7, 0 (baseline), 45 (mid-intervention), 84, and 91 (end-intervention). DHA supplementation for 45 d decreased (P < 0.05) fasting RLP-C (36%) and increased plasma eicosapentaenoic acid (EPA):arachidonic acid (AA) (100%) and the RBC (n-3) index (109%). Continued supplementation with DHA between d 45 and 91 further increased the RBC (n-3) index (162%) and plasma EPA:AA (137%) compared with baseline values. RLP-C concentration was positively associated (P < 0.01) with the plasma concentrations of triacylglycerols (Kendall's correlation coefficient or r = 0.46), triacylglycerol:HDL cholesterol (HDL-C) (r = 0.44), total cholesterol:HDL-C (r = 0.26), Apo B (r = 0.22), C III (r = 0.41), and E (r = 0.17), and 18:1(n-9) (r = 0.32); it was negatively associated (P < 0.05) with plasma concentrations of DHA (r = -0.32), EPA (r = -0.25), HDL-C (r = -0.21), LDL cholesterol:Apo B (r = -0.30), and HDL-C:Apo A (r = -0.25). Supplementation with placebo oil did not alter any of the response variables tested. Decreased atherogenic RLP-C and increased cardio-protective (n-3) index may improve cardio-vascular health.
血浆残留样颗粒胆固醇（RLP-C）和红细胞（n-3）指数是心血管疾病的新型危险因素。二十二碳六烯酸（DHA）补充剂对高甘油三酯血症男性这些危险因素的影响尚未得到研究。我们确定了DHA补充剂对血浆RLP-C浓度、红细胞（n-3）指数以及血浆RLP-C浓度与血浆脂质和脂肪酸浓度之间关联的影响。39至66岁的高甘油三酯血症男性参与了一项随机、安慰剂对照、平行研究。他们在8天内不服用补充剂，然后在最后90天内每天服用7.5克DHA油（3克DHA/天）或橄榄油（安慰剂）。在研究第-7天、0天（基线）、45天（干预中期）、84天和91天（干预结束）采集空腹血样。补充DHA 45天可降低（P<0.05）空腹RLP-C（36%），并增加血浆二十碳五烯酸（EPA）：花生四烯酸（AA）（100%）和红细胞（n-3）指数（109%）。与基线值相比，在第45天至91天继续补充DHA可进一步提高红细胞（n-3）指数（162%）和血浆EPA：AA（137%）。RLP-C浓度与血浆甘油三酯浓度（肯德尔相关系数或r = 0.46）、甘油三酯：高密度脂蛋白胆固醇（HDL-C）（r = 0.44）、总胆固醇：HDL-C（r = 0.26）、载脂蛋白B（r = 0.22）、C III（r = 0.41）和E（r = 0.17）以及18:1（n-9）（r = 0.32）呈正相关（P<0.01）；与血浆DHA（r = -0.32）、EPA（r = -0.25）、HDL-C（r = -0.21）、低密度脂蛋白胆固醇：载脂蛋白B（r = -0.30）和HDL-C：载脂蛋白A（r = -0.25）浓度呈负相关（P<0.05）。补充安慰剂油未改变任何测试的反应变量。降低致动脉粥样硬化的RLP-C并增加心脏保护（n-3）指数可能改善心血管健康。
