Razani Seyed Hadi, Mousavi Seyed Mohammad, Yeganeh Hassan Mehrabani Mehrabanii, Marc Ivan
Department of Food Science & Engineering, Faculty of Biosystem Engineering, University of Tehran, Karaj, Iran.
J Microbiol Biotechnol. 2007 Oct;17(10):1591-7.
The production of carotenoids, lipid content, and fatty acid composition were all studied in a strain of Sporobolomyces ruberrimus when using different concentrations of technical glycerol as the carbon source and ammonium sulfate as the nitrogen source. The total lipids represented an average of 13% of the dry weight, and the maximum lipids were obtained when using 65.5 g/l technical glycerol (133.63 mg/ g). The optimal conditions for fatty acid production were at 27 degrees C using 20 g of ammonium sulfate and a pH range from 6 to 7, which produced a fatty acid yield of 32.5+/-1 mg/g, including 1.27+/- 0.15 mg of linolenic acid (LNA), 7.50+/-0.45 mg of linoleic acid (LLA), 5.50+/-0.35 mg of palmitic acid (PA), 0.60+/-0.03 mg of palmitoleic acid (PAL), 1.28+/-0.11 mg of stearic acid (SA), 9.09+/-0.22 mg of oleic acid, 2.50+/-0.10 mg of erucic acid (EA), and 4.25+/-0.20 mg of lignoceric acid (LCA), where the palmitic, oleic, and linoleic acids combined formed about 37% of the total fatty acids. The concentration of total carotenoids was 2.80 mg/g when using 20 g of ammonium sulfate, and consisted of torularhodin (2.70 mg/g) and beta-carotene (0.10 mg/ g), at 23 degrees C and pH 6. However, the highest amount with the maximum specific growth rate was obtained (micromax=0.096 h(-1)) with an ammonium sulfate concentration of 30 g/l.
以不同浓度的工业甘油作为碳源、硫酸铵作为氮源，对深红掷孢酵母菌株的类胡萝卜素产量、脂质含量和脂肪酸组成进行了研究。总脂质平均占干重的13%，使用65.5 g/l工业甘油时脂质含量最高（133.63 mg/g）。脂肪酸产生的最佳条件是在27℃、使用20 g硫酸铵且pH值范围为6至7，此时脂肪酸产量为32.5±1 mg/g，其中包括1.27±0.15 mg的亚麻酸（LNA）、7.50±0.45 mg的亚油酸（LLA）、5.50±0.35 mg的棕榈酸（PA）、0.60±0.03 mg的棕榈油酸（PAL）、1.28±0.11 mg的硬脂酸（SA）、9.09±0.22 mg的油酸、2.50±0.10 mg的芥酸（EA）和4.25±0.20 mg的木蜡酸（LCA），其中棕榈酸、油酸和亚油酸合起来约占总脂肪酸的37%。使用20 g硫酸铵时，在23℃和pH值为6的条件下，总类胡萝卜素浓度为2.80 mg/g，由球红酵母红素（2.70 mg/g）和β-胡萝卜素（0.10 mg/g）组成。然而，硫酸铵浓度为30 g/l时，获得了最高产量以及最大比生长速率（μmax = 0.096 h-1）。
