Lee Jaslyn Jie Lin, Chen Liwei, Shi Jiahua, Trzcinski Antoine, Chen Wei-Ning
Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University , 1 Cleantech Loop, Singapore 637141.
J Agric Food Chem. 2014 Oct 15;62(41):10203-9. doi: 10.1021/jf502987q. Epub 2014 Oct 6.
Carotenoid production from three strains of Rhodosporidium toruloides grown on glycerol was studied. A time-dependent metabolomics approach was used to understand its metabolism on glycerol and mechanism for carotenoid production in three strains during different growth phases (1, 4, 7, and 12 days). Strain CBS 5490 was the highest carotenoid producer (28.5 mg/L) and had a unique metabolic profile. In this strain, metabolites belonging to the TCA cycle and amino acids were produced in lower amounts, as compared to the other strains. On the other hand, it produced the highest amounts of carotenoid and fatty acid metabolites. This indicated that the lower production of the TCA cycle and amino acid metabolites promoted energy and metabolic flux toward the carotenoid and fatty acid synthesis metabolic pathways. This study shows that metabolomic profiling is a useful tool to gain insight into the metabolic pathways in the cell and to shed light on the different molecular mechanisms between strains.
研究了三株红酵母在甘油上生长时类胡萝卜素的产生情况。采用时间依赖性代谢组学方法，以了解其在甘油上的代谢以及三株菌株在不同生长阶段（1、4、7和12天）产生类胡萝卜素的机制。CBS 5490菌株是最高的类胡萝卜素生产者（28.5 mg/L），具有独特的代谢谱。与其他菌株相比，该菌株中属于三羧酸循环和氨基酸的代谢物产生量较低。另一方面，它产生的类胡萝卜素和脂肪酸代谢物量最高。这表明三羧酸循环和氨基酸代谢物的较低产生促进了能量和代谢通量向类胡萝卜素和脂肪酸合成代谢途径的流动。这项研究表明，代谢组学分析是深入了解细胞代谢途径以及揭示菌株间不同分子机制的有用工具。
