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Presence of side-population cells in an immortalized nontumorigenic human liver epithelial cell line.

作者信息

Yamazaki Taisuke, Enosawa Shin, Tsukiyama Takashi, Tokiwa Takayoshi

机构信息

Department of Liver Cell Biology, Kohno Clinical Medicine Research Institute, 1-28-15 Kitashinagawa, Shinagawa, Tokyo, Japan.

出版信息

In Vitro Cell Dev Biol Anim. 2008 Jan-Feb;44(1-2):6-9. doi: 10.1007/s11626-007-9071-z. Epub 2007 Dec 22.

DOI:10.1007/s11626-007-9071-z
PMID:18157683
Abstract

Side-population (SP) cells have been shown to be highly enriched stem cells. We investigated whether an immortalized, nontumorigenic human liver cell line, THLE-5b, contains SP cells. Flow cytometry analysis after Hoechst 33342 staining demonstrated that the THLE-5b line contained a small component of SP cells. These SP cells were essentially eliminated by treatment with verapamil and expressed higher levels of ABCG2 mRNA than non-SP cells. In addition, the level of these SP cells detected by Hoechst 33342 staining was affected by the experimental conditions including the incubation medium. This is the first report of the presence of SP cells in the immortalized, nontumorigenic human liver cell line.

摘要

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引用本文的文献

1
Side Population Cells From an Immortalized Human Liver Epithelial Cell Line Exhibit Hepatic Stem-Like Cell Properties.来自永生化人肝上皮细胞系的侧群细胞表现出肝干细胞样细胞特性。
Cell Med. 2012 Jun 15;3(1-3):127-135. doi: 10.3727/215517912X639450. eCollection 2012 Jan.
2
Characterisation of human limbal side population cells isolated using an optimised protocol from an immortalised epithelial cell line and primary limbal cultures.采用改良方案从永生化上皮细胞系和原代角膜缘培养物中分离的人角膜缘侧群细胞的特征。
Stem Cell Rev Rep. 2014 Apr;10(2):240-50. doi: 10.1007/s12015-013-9481-0.

本文引用的文献

1
Cloning and characterization of liver progenitor cells from the scattered cell clusters in primary culture of porcine livers.从猪肝脏原代培养的散在细胞簇中克隆并鉴定肝祖细胞。
Cell Transplant. 2008;17(1-2):179-86. doi: 10.3727/000000008783907080.
2
Differentiation potential of an immortalized non-tumorigenic human liver epithelial cell line as liver progenitor cells.一种永生化非致瘤性人肝上皮细胞系作为肝祖细胞的分化潜能。
Cell Biol Int. 2006 Dec;30(12):992-8. doi: 10.1016/j.cellbi.2006.07.006. Epub 2006 Aug 7.
3
Side population purified from hepatocellular carcinoma cells harbors cancer stem cell-like properties.
从肝癌细胞中纯化出的侧群细胞具有癌干细胞样特性。
Hepatology. 2006 Jul;44(1):240-51. doi: 10.1002/hep.21227.
4
Characterization of a side population of cancer cells from human gastrointestinal system.人类胃肠道系统癌细胞侧群的特征分析。
Stem Cells. 2006 Mar;24(3):506-13. doi: 10.1634/stemcells.2005-0282. Epub 2005 Oct 20.
5
Side population cells derived from adult human liver generate hepatocyte-like cells in vitro.源自成人肝脏的侧群细胞可在体外生成类肝细胞。
Dig Dis Sci. 2005 Oct;50(10):1755-63. doi: 10.1007/s10620-005-2933-x.
6
Hepatic oval cells have the side population phenotype defined by expression of ATP-binding cassette transporter ABCG2/BCRP1.肝卵圆细胞具有由ATP结合盒转运体ABCG2/BCRP1的表达所定义的侧群表型。
Am J Pathol. 2003 Jul;163(1):3-9. doi: 10.1016/S0002-9440(10)63624-3.
7
Colony-forming capacity of porcine liver epithelial cells in culture.培养的猪肝脏上皮细胞的集落形成能力。
In Vitro Cell Dev Biol Anim. 2002 Sep;38(8):440-2. doi: 10.1290/1071-2690(2002)038<0440:CFCOPL>2.0.CO;2.
8
The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype.ABC转运蛋白Bcrp1/ABCG2在多种干细胞中表达，是旁群细胞表型的分子决定因素。
Nat Med. 2001 Sep;7(9):1028-34. doi: 10.1038/nm0901-1028.
9
The in vitro differentiating capacity of nonparenchymal epithelial cells derived from adult porcine livers.成年猪肝脏来源的非实质上皮细胞的体外分化能力。
Am J Pathol. 2000 Jun;156(6):2033-43. doi: 10.1016/S0002-9440(10)65075-4.
10
Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo.体内正在进行复制的小鼠造血干细胞的分离及其功能特性
J Exp Med. 1996 Apr 1;183(4):1797-806. doi: 10.1084/jem.183.4.1797.