Forest David L, Lindsay Sara M
School of Marine Sciences, University of Maine, Orono, Maine 04669, USA.
J Morphol. 2008 May;269(5):544-51. doi: 10.1002/jmor.10605.
Evidence suggests that ciliated sensory structures on the feeding palps of spionid polychaetes may function as chemoreceptors to modulate deposit-feeding activity. To investigate the probable sensory nature of these ciliated cells, we used immunohistochemistry, epi-fluorescence, and confocal laser scanning microscopy to label and image sensory cells, nerves, and their organization relative to the anterior central nervous system in several spionid polychaete species. Antibodies directed against acetylated alphatubulin were used to label the nervous system and detail the innervation of palp sensory cells in all species. In addition, the distribution of serotonin (5-HT) and FMRFamide-like immunoreactivity was compared in the spionid polychaetes Dipolydora quadrilobata and Pygospio elegans. The distribution of serotonin immunoreactivity was also examined in the palps of Polydora cornuta and Streblospio benedicti. Serotonin immunoreactivity was concentrated in cells underlying the food groove of the palps, in the palp nerves, and in the cerebral ganglion. FMRFamide-like immunoreactivity was associated with the cerebral ganglia, nuchal organs and palp nerves, and also with the perikarya of ciliated sensory cells on the palps.
有证据表明，多毛纲矶沙蚕科动物取食触须上的纤毛感觉结构可能作为化学感受器来调节沉积取食活动。为了研究这些纤毛细胞可能的感觉特性，我们使用免疫组织化学、落射荧光和共聚焦激光扫描显微镜对几种矶沙蚕科多毛纲动物的感觉细胞、神经及其相对于前中枢神经系统的组织进行标记和成像。针对乙酰化α微管蛋白的抗体用于标记神经系统并详细显示所有物种触须感觉细胞的神经支配。此外，还比较了多毛纲矶沙蚕科动物四叶双齿围沙蚕和秀丽 pygospio 中血清素（5-HT）和 FMRF 酰胺样免疫反应性的分布。还研究了多齿围沙蚕和贝氏 Streblospio 触须中血清素免疫反应性的分布。血清素免疫反应性集中在触须食物沟下方的细胞、触须神经和脑神经节中。FMRF 酰胺样免疫反应性与脑神经节、颈器官和触须神经有关，也与触须上纤毛感觉细胞的核周体有关。
