Miron M J, Anctil M
Départment de Sciences Biologiques, Université de Montréal, Québec, Canada.
J Comp Neurol. 1988 Sep 15;275(3):429-40. doi: 10.1002/cne.902750307.
The distribution of serotoninergic neurons in the nervous system of the scale worm Harmothoe imbricata was visualized in the anterior half of the body by the peroxidase-antiperoxidase (PAP) immunohistochemical method with a specific antiserotonin antibody. Immunoreactive neuronal somata were localized in discrete ganglion cell masses of the dorsally situated cerebral ganglion and in segmental ganglia of the ventral nerve cord. They also make up the majority of neurons present in the parapodial ganglia. Large and small varicose fibers stained in the neuropile of all the above-mentioned ganglia but also in interganglionic connectives and segmental nerves. On the basis of soma size and location and of fiber distribution, the reactive neurons were identified as primarily interneuronal with a few motoneurons and presumptive afferent neurons. The presence of a motor component was substantiated by observations of several reactive varicose fibers spread over longitudinal muscle layers of the trunk. In addition, neurites of the subepidermal nerve plexus and enterochromaffinlike cells of the gut epithelium reacted with the serotonin antibody. It is concluded that serotoninergic pathways are ubiquitous elements in the organization of the central and peripheral nervous system of this polychaete. The significance of these findings in relation to other annelid groups and to the physiological role of serotonin is discussed.
采用过氧化物酶抗过氧化物酶（PAP）免疫组织化学方法，使用特异性抗血清素抗体，在鳞沙蚕（Harmothoe imbricata）身体前半部分可视化了血清素能神经元在神经系统中的分布。免疫反应性神经元胞体定位于背侧脑节的离散神经节细胞团以及腹神经索的节段神经节中。它们还构成了疣足神经节中大多数神经元。大小不一的曲张纤维在上述所有神经节的神经纤维网中染色，但在神经节间连接和节段神经中也有染色。根据胞体大小、位置和纤维分布，反应性神经元主要被鉴定为中间神经元，还有一些运动神经元和推测的传入神经元。通过观察几条分布在躯干纵肌层上的反应性曲张纤维，证实了运动成分的存在。此外，表皮下神经丛的神经突和肠上皮的肠嗜铬样细胞与血清素抗体发生反应。得出的结论是，血清素能通路是这种多毛纲动物中枢和外周神经系统组织中普遍存在的元素。讨论了这些发现与其他环节动物类群以及血清素生理作用相关的意义。
