Kim Ju-Young, Yoon Mi-Young, Cha Mi-Ran, Hwang Ji-Hwan, Park Eunju, Choi Sun-Uk, Park Hae-Ryong, Hwang Yong-Il
Department of Food Science and Biotechnology, Kyungnam University, Masan, Republic of Korea.
J Med Food. 2007 Dec;10(4):587-93. doi: 10.1089/jmf.2007.002.
Natural marine products have recently become the focus of increased research interest, due to their potential pharmacological activities. Therefore, we have screened 50 varieties of marine seaweed and determined that the methanolic extracts from Plocamium telfairiae (PTE) exhibited a cytotoxic effect against HT-29 human colon carcinoma cells. In this study, we report on the cytotoxic activity and mechanism of PTE-induced apoptosis in HT-29 cells. The treatment of HT-29 cells with various PTE concentrations resulted in the inhibition of growth and the induction of apoptosis in a dose-dependent manner, as determined by the results of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay, a lactate dehydrogenase release assay, a morphological assay, and a colony formation assay. Interestingly, we also detected apoptotic bodies on Hoechst staining and attempted to determine whether the PTE-induced apoptosis involved the caspase pathway, using a caspase colorimetric assay. The activation of caspases-8, -9, -3, and -7 and the specific proteolytic cleavage of poly(ADP-ribose) polymerase were detected over the course of apoptosis induction. Our results showed that PTE may function as a chemopreventive and/or chemotherapeutic agent in colon carcinoma cells via the reduction of cell viability and the induction of apoptosis.
由于天然海洋产物具有潜在的药理活性，它们最近已成为研究兴趣日益增加的焦点。因此，我们筛选了50种海洋海藻，并确定了来自泰氏蜈蚣藻（PTE）的甲醇提取物对HT-29人结肠癌细胞具有细胞毒性作用。在本研究中，我们报道了PTE诱导HT-29细胞凋亡的细胞毒性活性及机制。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐还原试验、乳酸脱氢酶释放试验、形态学试验和集落形成试验的结果确定，用不同浓度的PTE处理HT-29细胞导致生长抑制和凋亡诱导呈剂量依赖性。有趣的是，我们还在Hoechst染色中检测到凋亡小体，并试图使用半胱天冬酶比色法确定PTE诱导的凋亡是否涉及半胱天冬酶途径。在凋亡诱导过程中检测到半胱天冬酶-8、-9、-3和-7的激活以及聚（ADP-核糖）聚合酶的特异性蛋白水解切割。我们的结果表明，PTE可能通过降低细胞活力和诱导凋亡而在结肠癌细胞中发挥化学预防和/或化学治疗剂的作用。
