Somasekharan Syam Prakash, El-Naggar Amal, Sorensen Poul H, Wang Yuzhuo, Cheng Hongwei
Centre for Biosciences and Biomedical Engineering, Indian Institute of Technology, Indore, India.
Department of Molecular Oncology, British Columbia Cancer Research Centre, Vancouver, BC, Canada.
Evid Based Complement Alternat Med. 2016;2016:9730654. doi: 10.1155/2016/9730654. Epub 2016 Aug 31.
Research on marine natural products as potential anticancer agents is still limited. In the present study, an aqueous extract of a Canadian marine microalgal preparation was assessed for anticancer activities using various assays and cell lines of human cancers, including lung, prostate, stomach, breast, and pancreatic cancers, as well as an osteosarcoma. In vitro, the microalgal extract exhibited marked anticolony forming activity. In addition, it was more toxic, as indicated by increased apoptosis, to nonadherent cells (grown in suspension) than to adherent cells. In vivo, an antimetastatic effect of the extract was observed in NOD-SCID mice carrying subrenal capsule xenografts of PC3 prostate cancer cells. The results of the present study suggest that the antimetastatic effect of the aqueous microalgal extract is based on inhibition of colony forming ability of cancer cells and the preferential killing of suspended cancer cells. Further research aimed at identification of the molecular basis of the anticancer activities of the microalgal extract appears to be warranted.
将海洋天然产物作为潜在抗癌剂的研究仍然有限。在本研究中，使用包括肺癌、前列腺癌、胃癌、乳腺癌和胰腺癌以及骨肉瘤在内的各种人类癌症检测方法和细胞系，对一种加拿大海洋微藻制剂的水提取物的抗癌活性进行了评估。在体外，微藻提取物表现出显著的抗集落形成活性。此外，如细胞凋亡增加所示，它对非贴壁细胞（悬浮生长）的毒性比对贴壁细胞更大。在体内，在携带PC3前列腺癌细胞肾包膜下异种移植物的NOD-SCID小鼠中观察到该提取物的抗转移作用。本研究结果表明，微藻水提取物的抗转移作用基于对癌细胞集落形成能力的抑制和对悬浮癌细胞的优先杀伤。针对确定微藻提取物抗癌活性的分子基础的进一步研究似乎是必要的。
