Scheidt Holger A, Huster Daniel
Junior Research Group-Structural Biology of Membrane Proteins, Institute of Biochemistry/Biotechnology, Martin Luther University, Halle-Wittenberg, Halle, Germany.
Acta Pharmacol Sin. 2008 Jan;29(1):35-49. doi: 10.1111/j.1745-7254.2008.00726.x.
The interaction of small molecules with lipid membranes and the exact knowledge of their binding site and bilayer distribution is of great pharmacological importance and represents an active field of current biophysical research. Over the last decade, a highly resolved 1H solid-state NMR method has been developed that allows measuring localization and distribution of small molecules in membranes. The classical solution 1H NMR NOESY technique is applied to lipid membrane samples under magic-angle spinning (MAS) and NOESY cross-relaxation rates are determined quantitatively. These rates are proportional to the contact probability between molecular segments and therefore an ideal tool to study intermolecular interactions in membranes. Here, we review recent 1H MAS NOESY applications that were carried out to study lateral lipid organization in mixed membranes and the interaction of membranes with water, ethanol, small aromatic compounds, peptides, fluorescence labels, and lipophilic nucleosides.
小分子与脂质膜的相互作用以及对其结合位点和双层分布的确切了解具有重要的药理学意义，是当前生物物理研究的一个活跃领域。在过去十年中，已开发出一种高分辨率的1H固态核磁共振方法，可用于测量小分子在膜中的定位和分布。经典的溶液1H NMR NOESY技术应用于魔角旋转（MAS）下的脂质膜样品，并定量测定NOESY交叉弛豫率。这些速率与分子片段之间的接触概率成正比，因此是研究膜中分子间相互作用的理想工具。在此，我们综述了最近利用1H MAS NOESY开展的应用，这些应用旨在研究混合膜中的横向脂质组织以及膜与水、乙醇、小芳香化合物、肽、荧光标记物和亲脂性核苷的相互作用。
