Chang C W, Imai K, Chang Y C, Hayashi T, Kohno H, Nagasue N, Nakamura T
Department of Biochemistry, Shimane Medical University, Izumo, Japan.
Enzyme. 1991;45(3):145-54. doi: 10.1159/000468881.
During liver transplantation in the pig, the plasma activities of beta-galactosidase, beta-glucuronidase and beta-glucosidase were elevated as early as 15 min after establishing the hepatic circulation. The enzyme activities peaked at 3 h and returned to the initial level within 2-3 days. However, such substantial alterations were not observed in other enzymes, alpha-mannosidase and alpha-glucosidase. Similar reactions to those of the first three enzymes were found in aspartate aminotransferase and lactate dehydrogenase but with later peaks and slower eliminations. In light of the current study, the serial estimation of acid hydrolases may be useful to discover the extent of tissue injury and also to evaluate the effectiveness of various organ-preservation methods.
在猪肝脏移植过程中，早在肝循环建立后15分钟，β-半乳糖苷酶、β-葡萄糖醛酸酶和β-葡萄糖苷酶的血浆活性就升高了。酶活性在3小时达到峰值，并在2 - 3天内恢复到初始水平。然而，在其他酶（α-甘露糖苷酶和α-葡萄糖苷酶）中未观察到如此显著的变化。天冬氨酸转氨酶和乳酸脱氢酶也出现了与前三种酶类似的反应，但峰值出现较晚且消除较慢。根据当前的研究，对酸性水解酶进行连续测定可能有助于发现组织损伤的程度，也有助于评估各种器官保存方法的有效性。
