Wen Bixiu, Urano Muneyasu, Humm John L, Seshan Venkatraman E, Li Gloria C, Ling C Clifton
Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Radiat Res. 2008 Jan;169(1):67-75. doi: 10.1667/RR0888.1.
Wen, B., Urano, M., Humm, J. L., Seshan, V. E., Li, G. C. and Ling, C. C. Comparison of Helzel and OxyLite Systems in the Measurements of Tumor Partial Oxygen Pressure (pO(2)). Radiat. Res. 168, 67-75 (2008). It has been demonstrated in both experimental and human malignancies that hypoxic tumor cells are linked with aggressive disease phenotype. One of the methods to identify these cells is by direct physical measurement of tumor pO(2). This study compared pO(2) values measured with two systems, the Helzel Hypoximeter (successor of the polarographic Eppendorf electrode) and the Oxford-Optronix OxyLite (fiber-optic probe), in R3327-AT and R3327-AT/tkeGFP tumors. Partial oxygen pressure was measured in individual tumors with either system or in the same tumor with both systems. The similarities and discrepancies in pO(2) measurements between the two systems were also investigated when tumor-bearing animals were breathing pure oxygen. Our data showed a considerable heterogeneity in pO(2) values in each tumor using both the Helzel and OxyLite systems. Similar results were obtained with both systems for the mean and median pO(2) values, and the distributions of pO(2) values within the interval 0 < pO(2) < 40 mmHg (the range important for defining tumor hypoxia) were found to be statistically equivalent. However, the frequencies of high pO(2) values (>40 mmHg) and zero values measured by the two systems were statistically significantly different.
温，B.，浦野，M.，胡姆，J. L.，塞尚，V. E.，李，G. C.以及凌，C. C.。Helzel系统与OxyLite系统在肿瘤局部氧分压（pO₂）测量中的比较。《辐射研究》168卷，67 - 75页（2008年）。在实验性和人类恶性肿瘤中均已证明，缺氧肿瘤细胞与侵袭性疾病表型相关。识别这些细胞的方法之一是直接物理测量肿瘤pO₂。本研究比较了在R3327 - AT和R3327 - AT/tkeGFP肿瘤中，使用两种系统（Helzel血氧计（极谱式Eppendorf电极的后继产品）和牛津 - 奥普特尼克斯OxyLite（光纤探头））测量的pO₂值。用任一系统测量单个肿瘤的局部氧分压，或用两种系统测量同一肿瘤的局部氧分压。还研究了荷瘤动物呼吸纯氧时，两种系统在pO₂测量中的异同。我们的数据显示，使用Helzel和OxyLite系统时，每个肿瘤的pO₂值存在相当大的异质性。两种系统获得的pO₂均值和中位数结果相似，并且发现0 < pO₂ < 40 mmHg区间内（定义肿瘤缺氧的重要范围）的pO₂值分布在统计学上是等效的。然而，两种系统测量的高pO₂值（>40 mmHg）和零值的频率在统计学上有显著差异。
