Kim Myeong Hee, Choi Sung Eun, Oh Heung Bum
Department of Laboratory Medicine, Asan Medical Center and University of Ulsan College of Medicine, Seoul, Korea.
Korean J Lab Med. 2007 Dec;27(6):442-50. doi: 10.3343/kjlm.2007.27.6.442.
To monitor the performance of histocompatibility testing laboratories, HLA proficiency survey in Korea has been conducted biannually since 1996. In this report, we summarized the results of the surveys performed in recent two years (2005-2006).
A total of four proficiency surveys were performed, in which 59-61 laboratories participated. Each survey included three tests for HLA class I (serology and DNA) and class II (DNA) typing and six tests for HLA crossmatch.
The overall concordance of serologic typing was 98.9% (355/359) for HLA-A, 97.5% (350/ 359) for HLA-B, and 94.7% (337/356) for HLA-C. The antigens assigned correctly by less than 95% of the participating laboratories were A26 (93.8%), B38 (94.2%), Cw3/Cw10 (90.9%), Cw6 (94.4%), and Cw8 (74.3%). The overall concordance rates of DNA typing were 99.6% (533/535) for HLA-A, 99.8% (539/540) for HLA-B, and 100% (392/392) for HLA-C. Correct assignment of HLA-DRB1 and -DQB1 was reported by 99.2% (98.1-100%) and 96.7% (88.9-100%) for the generic level and 100% and 95.8% (75-100%) for the allelic level, respectively. On the average 3.8% (0-7.7%) of the total laboratories showed unacceptable results in the crossmatch tests.
The rates of correct antigen identification and of unacceptable crossmatch were similar to those of previous surveys, which were considered satisfactory. The Korean proficiency survey program may have contributed to a high quality of HLA tests today and should be continued for further improvements of the tests tomorrow.
为监测组织相容性检测实验室的表现，自1996年起韩国每两年进行一次HLA能力验证调查。在本报告中，我们总结了最近两年（2005 - 2006年）的调查结果。
共进行了四次能力验证调查，有59 - 61个实验室参与。每次调查包括三项HLAⅠ类（血清学和DNA）及Ⅱ类（DNA）分型检测，以及六项HLA交叉配型检测。
血清学分型的总体一致性在HLA - A为98.9%（355/359），HLA - B为97.5%（350/359），HLA - C为94.7%（337/356）。参与实验室中不到95%正确分型的抗原为A26（93.8%）、B38（94.2%）、Cw3/Cw10（90.9%）、Cw6（94.4%）和Cw8（74.3%）。DNA分型的总体一致性在HLA - A为99.6%（533/535），HLA - B为99.8%（539/540），HLA - C为100%（392/392）。HLA - DRB1和 - DQB1在通用水平的正确分型报告率分别为99.2%（98.1 - 100%）和96.7%（88.9 - 100%），在等位基因水平分别为100%和95.8%（75 - 100%）。平均有3.8%（0 - 7.7%）的实验室在交叉配型检测中结果不合格。
正确抗原识别率和交叉配型不合格率与以往调查相似，被认为是令人满意的。韩国的能力验证调查项目可能有助于如今高质量的HLA检测，并且应该继续开展以在未来进一步改进检测。
