Claessens An, Weyn Christine, Merregaert Joseph
Laboratory of Molecular Biotechnology, Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, B-2610, Wilrijk, Belgium.
Cell Mol Biol Lett. 2008;13(2):250-9. doi: 10.2478/s11658-007-0052-8. Epub 2008 Apr 10.
Mouse chondrolectin (chodl) was isolated out of the tail tip of four-day old 129/SvJ mice as a by-product of a PCR-based subtractive cDNA library screening. The gene is predominantly expressed in adult skeletal muscle, heart, testes and lungs and in embryonic stadia. Chodl is the mouse homologue of human chondrolectin (CHODL), a gene that encodes for a type Ia transmembrane protein and that is expressed in human testis, prostate, heart and skeletal muscle tissue. CHODL-splice variants (CHODL ( f ), CHODL ( f Delta E ), CHODL ( Delta E )) are detected in human leukocytes. The proteins of the chondrolectin family belong to the family of C-type lectins. As the members of this protein family are important for a wide array of biological processes, the function of chodl was investigated by searching for its protein interaction partners. The beta-subunit of Rab geranylgeranyl transferase (Rabggtb) was isolated 8 times after a complete Sos recruitment system (SRS) screen with the cytoplasmic domain of chodl. The interaction was confirmed with in vitro transcription/translation and co-immunoprecipitation (co-IP) experiments.
小鼠软骨凝集素(chodl)是在对4日龄129/SvJ小鼠的尾尖进行基于聚合酶链反应(PCR)的消减cDNA文库筛选时作为副产品分离出来的。该基因主要在成年骨骼肌、心脏、睾丸和肺以及胚胎期表达。Chodl是人类软骨凝集素(CHODL)的小鼠同源物,CHODL基因编码一种Ia型跨膜蛋白,在人类睾丸、前列腺、心脏和骨骼肌组织中表达。在人类白细胞中检测到CHODL剪接变体(CHODL(f)、CHODL(fΔE)、CHODL(ΔE))。软骨凝集素家族的蛋白质属于C型凝集素家族。由于该蛋白质家族的成员对广泛的生物过程很重要,因此通过寻找其蛋白质相互作用伙伴来研究chodl的功能。在用chodl的细胞质结构域进行完整的Sos募集系统(SRS)筛选后,Rab geranylgeranyl转移酶(Rabggtb)的β亚基被分离出8次。通过体外转录/翻译和免疫共沉淀(co-IP)实验证实了这种相互作用。